Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Pareja-Rivera, Carina; Cuéllar-Cruz, Mayra; Esturau-Escofet, Nuria; Demitri, Nicola; Polentarutti, Maurizio; Stojanoff, Vivian; Moreno, Abel

doi:10.1021/acs.cgd.6b01362

Title: Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Journal Article · Mon Dec 05 00:00:00 EST 2016 · Crystal Growth and Design

DOI:https://doi.org/10.1021/acs.cgd.6b01362· OSTI ID:1392208

Pareja-Rivera, Carina ^[1]; Cuéllar-Cruz, Mayra ^[2]; Esturau-Escofet, Nuria ^[1];

^[3]; Polentarutti, Maurizio ^[3]; Stojanoff, Vivian ^[4];

^[1]

National Autonomous University of Mexico (Mexico). Institute of Chemistry
University of Guanajuato (Mexico). Department of Biology, Division of Natural and Exact Sciences
Elettra Sincrotrone Trieste (Italy)
Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source

Here, in this contribution we use nonconventional methods that help to increase the success rate of a protein crystal growth, and consequently of structural projects using X-ray diffraction techniques. In order to achieve this purpose, this contribution presents new approaches involving more sophisticated techniques of protein crystallization, not just for growing protein crystals of different sizes by using electric fields, but also for controlling crystal size and orientation. Also, this latter was possible through the use of magnetic fields that allow to obtain protein crystals suitable for both high-resolution X-ray and neutron diffraction crystallography where big crystals are required. This contribution discusses some pros, cons and realities of the role of electromagnetic fields in protein crystallization research, and their effect on protein crystal contacts. Additionally, we discuss the importance of room and low temperatures during data collection. Finally, we also discuss the effect of applying a rather strong magnetic field of 16.5 T, for shorts and long periods of time, on protein crystal growth, and on the 3D structure of two model proteins.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704; AC02-76SF00515

OSTI ID:: 1392208

Report Number(s):: BNL-113790-2017-JA

Journal Information:: Crystal Growth and Design, Vol. 17, Issue 1; ISSN 1528-7483

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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36 MATERIALS SCIENCE
59 BASIC BIOLOGICAL SCIENCES
electric fields
magnetic fields
lysozyme
glucose isomerase
crystal growth
in situ data collection
protein crystallization

Title: Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

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Cited By (7)

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