Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays

Ekman, Axel A.; Chen, Jian-Hua; Guo, Jessica; McDermott, Gerry; Le Gros, Mark A.; Larabell, Carolyn A.

doi:10.1111/boc.201600044

Title: Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell: Mesoscale imaging with cryo-light and X-rays

Journal Article · Mon Nov 14 00:00:00 EST 2016 · Biology of the Cell

DOI:https://doi.org/10.1111/boc.201600044· OSTI ID:1379651

Ekman, Axel A. ^[1]; Chen, Jian-Hua ^[1]; Guo, Jessica ^[1]; McDermott, Gerry ^[1]; Le Gros, Mark A. ^[2]; Larabell, Carolyn A. ^[2]

Univ. of California, San Francisco, CA (United States)
Univ. of California, San Francisco, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self‐organise to form large, functional structures. A comprehensive set of rules governing mesoscale self‐organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X‐ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high‐resolution structural information with precise molecular localisation data.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; DE‐AC02‐05CH11231

OSTI ID:: 1379651

Alternate ID(s):: OSTI ID: 1401243

Journal Information:: Biology of the Cell, Vol. 109, Issue 1; ISSN 0248-4900

Country of Publication:: United States

Language:: English

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Cited by: 23 works

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