In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel
Abstract
Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1367386
- Report Number(s):
- PNNL-SA-109594
Journal ID: ISSN 0003-2654; 48569; 49694
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Analyst
- Additional Journal Information:
- Journal Volume: 142; Journal Issue: 13; Journal ID: ISSN 0003-2654
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- SALVI; microfluidics; diffusion coefficient; relaxometry; MRI; nuclear magnetic resonance; biofilm; Shewanella; Environmental Molecular Sciences Laboratory
Citation Formats
Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., and Yu, X. -Y. In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel. United States: N. p., 2017.
Web. doi:10.1039/C7AN00078B.
Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., & Yu, X. -Y. In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel. United States. https://doi.org/10.1039/C7AN00078B
Renslow, R. S., Marshall, M. J., Tucker, A. E., Chrisler, W. B., and Yu, X. -Y. 2017.
"In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel". United States. https://doi.org/10.1039/C7AN00078B.
@article{osti_1367386,
title = {In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel},
author = {Renslow, R. S. and Marshall, M. J. and Tucker, A. E. and Chrisler, W. B. and Yu, X. -Y.},
abstractNote = {Nuclear magnetic resonance (NMR) microimaging and spectroscopy was used to interrogate fluids of biological importance (e.g., water, buffer, medium solution) and live biofilms in a microchannel compatible for analyses at ambient pressure and under vacuum. Studies using buffer, growth medium, and actively growing Shewanella oneidensis biofilms were used to demonstrate in situ NMR microimaging measurement capabilities including velocity mapping, diffusion coefficient mapping, relaxometry, localized spectroscopy, and 2D and 3D imaging within a microchannel suitable for different analytical platforms. This technique is promising for diverse applications of correlative imaging using a portable microfluidic platform.},
doi = {10.1039/C7AN00078B},
url = {https://www.osti.gov/biblio/1367386},
journal = {Analyst},
issn = {0003-2654},
number = 13,
volume = 142,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2017},
month = {Sun Jan 01 00:00:00 EST 2017}
}
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