DNA repair by UvrABC: In vitro analysis of the preincision complex
- Gorlaeus Labs., Leiden (Netherlands); and others
The UvrABC endonuclease repairs a variety of structurally different lesions. The preincision complex consisting of UvrB and damaged DNA plays a central role in successful damage recognition by UvrA and UvrB, and is a prerequisite for the subsequent processing in the presence of UvrC, resulting in dual incisions of the DNA. Although there is still an effect on efficiency, the type of lesion seems to have only a minor influence on the mechanism of repair, since incisions occur at fixed distances from each lesion. Therefore, an important aspect of unraveling the molecular mechanisms of UvrABC-dependent DNA repair is to understand how this system is able to process different lesions in a similar way. Attention in this work has been focused on which domains of UvrB are specifically involved in the formation of the UvrB-DNA preincision complex and on the structure of this complex.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134868
- Report Number(s):
- CONF-9307221-; TRN: 95:007741-0035
- Resource Relation:
- Conference: DNA damage: effects on DNA structure and protein recognition, Burlington, VT (United States), 31 Jul - 4 Aug 1993; Other Information: PBD: 1994; Related Information: Is Part Of DNA damage: Effects on DNA structure and protein recognition; Wallace, S.S.; Van Houten, B.; Kow, Yoke Wah [eds.]; PB: 395 p.
- Country of Publication:
- United States
- Language:
- English
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