Differential Regulation of Duplicate Light-Dependent Protochlorophyllide Oxidoreductases in the Diatom Phaeodactylum tricornutum

Hunsperger, Heather M.; Ford, Christopher J.; Miller, James S.; Cattolico, Rose Ann; Ianora, ed., Adrianna

doi:10.1371/journal.pone.0158614

Title: Differential Regulation of Duplicate Light-Dependent Protochlorophyllide Oxidoreductases in the Diatom Phaeodactylum tricornutum

Journal Article · Fri Jul 01 00:00:00 EDT 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0158614· OSTI ID:1346608

Hunsperger, Heather M.; Ford, Christopher J.; Miller, James S.; Cattolico, Rose Ann; Ianora, ed., Adrianna

Diatoms (Bacilliariophyceae) encode two light-dependent protochlorophyllide oxidoreductases (POR1 and POR2) that catalyze the penultimate step of chlorophyll biosynthesis in the light. Algae live in dynamic environments whose changing light levels induce photoacclimative metabolic shifts, including altered cellular chlorophyll levels. We hypothesized that the two POR proteins may be differentially adaptive under varying light conditions. Using the diatom Phaeodactylum tricornutum as a test system, differences in POR protein abundance and por gene expression were examined when this organism was grown on an alternating light:dark cycles at different irradiances; exposed to continuous light; and challenged by a significant decrease in light availability. As a result, for cultures maintained on a 12h light: 12h dark photoperiod at 200μEm^–2 s^–1 (₂₀₀L/D), both por genes were up-regulated during the light and down-regulated in the dark, though por1 transcript abundance rose and fell earlier than that of por2. Little concordance occurred between por1 mRNA and POR1 protein abundance. In contrast, por2 mRNA and POR2 protein abundances followed similar diurnal patterns. When 200L/D P. tricornutum cultures were transferred to continuous light (₂₀₀L/L), the diurnal regulatory pattern of por1 mRNA abundance but not of por2 was disrupted, and POR1 but not POR2 protein abundance dropped steeply. Under 1200μEm^–2 s^–1 (₁₂₀₀L/D), both por1 mRNA and POR1 protein abundance displayed diurnal oscillations. A compromised diel por2 mRNA response under 1200L/D did not impact the oscillation in POR2 abundance. When cells grown at ₁₂₀₀L/D were then shifted to 50μEm^–2 s^–1 (50L/D), por1 and por2 mRNA levels decreased swiftly but briefly upon light reduction. Thereafter, POR1 but not POR2 protein levels rose significantly in response to this light stepdown.

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Research Organization:: Texas A&M AgriLife Research, College Station, TX (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: EE0003046

OSTI ID:: 1346608

Alternate ID(s):: OSTI ID: 1312900

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 11 Journal Issue: 7; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 4 works

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Title: Differential Regulation of Duplicate Light-Dependent Protochlorophyllide Oxidoreductases in the Diatom Phaeodactylum tricornutum

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