Molecular characterization of a human gene for S28 ribosomal binding protein

Journal Article · Thu Sep 01 00:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:134644

Wong, P; Borst, D E; Chader, G J ^[1]

---

1. National Eye Institute, Bethesda, MD (United States)

The mechanism of ribosome action and the ribosomal binding proteins which cooperatively interact in the working of this structure are not completely understood. Theoretically, mutations in genes that encode these proteins may compromise the efficiency of protein synthesis and therefore lead to a functional disorder. In the course of our search for human genes which show homology to the C. elegans CED-4 death gene, we have serendipitously identified one of the human S28 ribosomal binding protein genes as a random fragment fused to the end of one of our putative CED-4 positive homologue clones. The cloned S28 fragment consists of 381 nucleotides with a putative open reading frame of 113 amino acids. Sequence comparisons to GenBank revealed significant homologies to ribosomal binding protein genes in other species (including the rat S28 ribosomal binding protein gene) indicating that the S28 gene sequence is highly conserved. This finding is confirmed by zooblot analysis. Significant homologies also exist to two human expressed tagged sites (HUMRIBPROB; L05091 and HSAFIF072; Z21908). Analysis of the putative S28 peptide sequence allows insights into possible functional regions of the protein. The identification of 8 distinct bands upon Southern analysis of the S28 fragments suggests that there are multiple copies of the S28 gene in the human genome. Mapping of the S28 fragment on somatic cell hybrid panels identified distinct S28 gene loci on chromosomes 1, 2, 7, 10, 11, 12, 17 expression in adult tissues (pancreas, kidney, muscle, liver, lung, placenta, brain, heart, and retina) as well as in fetal tissues (kidney, liver, lung, brain, and heart).

• OSTI ID: 134644
• Report Number(s): CONF-941009-; ISSN 0002-9297; TRN: 95:005313-1381
• Journal Information: American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
RIBOSOMES
GENES
GENE MUTATIONS
DNA-CLONING
GENETIC MAPPING
DNA SEQUENCING
PROTEINS
SYNTHESIS
STRUCTURE-ACTIVITY RELATIONSHIPS
NEMATODES
GENETICS
COMPARATIVE EVALUATIONS
HUMAN POPULATIONS
SOMATIC CELLS
HYBRIDIZATION
HUMAN CHROMOSOMES
AMINO ACIDS
NUCLEOTIDES
PEPTIDES