Human expressed tagged sites on the X chromosome: A mapping resource for heritable sex-linked chorioretinal disorders
- Univ. of Alberta, Edmonton (Canada)
- National Eye Institute, Bethesda, MD (United States)
We have isolated a bank of human X-specific genomic clones which harbor chorioretinal expressed sequences using library to library cross-screening. The steps included (1) the creation of a {lambda}gt-10 library of human chorioretinal cDNA, (2) the creation of a human X-specific EMBL-3 genomic library from a somatic cell hybrid (82082a) containing the X chromosome as its only human component and lacking the hamster X, and (3) a PCR-based cross-screen to identify 78 clones expressed in choroid and retina. The characterization of one human X-specific EMBL-3 clone (XEH.8; DXS542) has provided a clear illustration of the feasibility of this approach. FISH mapping confirms the regional localization of XEH.8 to Xp11. Localization of additional clones, XEH.1, XEH.34, XEH.41, and XEH.52 will be presented along with partial sequencing and characterization. Our approach has focused on the search for expressed sequences which can serve as expressed tagged sites (ESTs) in mapping or candidate genes for heritable eye disorders.
- OSTI ID:
- 134442
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-1176
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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