Development of a single-cell X-ray fluorescence flow cytometer

Crawford, Andrew M.; Kurecka, Patrick; Yim, Tsz Kwan; Kozemchak, Claire; Deb, Aniruddha; Dostal, Lubomir; Sun, Cheng -Jun; Brewe, Dale L.; Barrea, Raul; Penner-Hahn, James E.

doi:10.1107/S1600577516008006

Title: Development of a single-cell X-ray fluorescence flow cytometer

Journal Article · Fri Jun 17 00:00:00 EDT 2016 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/S1600577516008006· OSTI ID:1339965

Crawford, Andrew M. ^[1]; Kurecka, Patrick ^[1]; Yim, Tsz Kwan ^[1]; Kozemchak, Claire ^[1]; Deb, Aniruddha ^[1]; Dostal, Lubomir ^[1]; Sun, Cheng -Jun ^[2]; Brewe, Dale L. ^[2]; Barrea, Raul ^[3]; Penner-Hahn, James E. ^[1]

Univ. of Michigan, Ann Arbor, MI (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)
DePaul Univ., Chicago, IL (United States)

An X-ray fluorescence flow cytometer that can determine the total metal content of single cells has been developed. Capillary action or pressure was used to load cells into hydrophilic or hydrophobic capillaries, respectively. Once loaded, the cells were transported at a fixed vertical velocity past a focused X-ray beam. X-ray fluorescence was then used to determine the mass of metal in each cell. By making single-cell measurements, the population heterogeneity for metals in the µM to mM concentration range on fL sample volumes can be directly measured, a measurement that is difficult using most analytical methods. This approach has been used to determine the metal composition of 936 individual bovine red blood cells (bRBC), 31 individual 3T3 mouse fibroblasts (NIH3T3) and 18 Saccharomyces cerevisiae (yeast) cells with an average measurement frequency of ~4 cells min^–1. These data show evidence for surprisingly broad metal distributions. Lastly, details of the device design, data analysis and opportunities for further sensitivity improvement are described.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH) - National Institute of General Medical Sciences; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1339965

Journal Information:: Journal of Synchrotron Radiation (Online), Vol. 23, Issue 4; ISSN 1600-5775

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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Cited By (1)

M-BLANK : a program for the fitting of X-ray fluorescence spectra Crawford, Andrew M.; Deb, Aniruddha; Penner-Hahn, James E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s1600577519000651/rv5095sup1.pdf	text	January 2019

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Title: Development of a single-cell X-ray fluorescence flow cytometer

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