Fluorescence in situ hybridization (FISH) study of response to granulocyte colony stimulating factor (G-CSF) in myelodysplasia associated with monosomy 7: Evidence for differentiation of the dysplastic clone
- Rhode Island Hospital, Providence, RI (United States)
- Brown Univ. School of Medicine, Providence, RI (United States)
Myelodysplastic syndromes (MDS) are clonal disorders of hematopoietic differentiation which may respond to the administration of cytokines with increases in the number of circulating mature neutrophils. We used fluorescence in situ hybridization (FISH) to investigate a young patient with myelodyplasia associated with monosomy 7 to determine whether mature appearing polymorphonuclear cells present in response to treatment with granulocyte colony stimulating factor (G-CSF) were progeny of the dysplastic clone or represented stimulation of residual normal hematopoiesis. The patient is a 26 year old male with a long history of a complex stem cell disorder dating to age 5. A chromosome fragility test was negative. In October 1993 treatment was begun with G-CSF when the absolute neutrophil count (ANC) fell to 300/mm{sup 3} despite GM-CSF therapy. Cytogenetic study of bone marrow just prior to starting G-CSF revealed monosomy 7 in all metaphases. A study in July, 1992 was normal. On his most recent marrow examination (1/94), blasts had numerous dysplastic forms. FISH was performed on buffy coat smears of patient and control specimens using a biotin labelled alpha satellite probe to chromosome 7. At the time of study, the peripheral blood count was 12,500 mm{sup 3}, with 56% neutrophils, 6% bands and no circulating blasts. Cells were scored as either polymorphonuclear or mononuclear cells. In a healthy control, 22 of 190 scored as either polymorphonuclear cells (12%) contained one chromosome 7 signal, versus 193 of 200 (96.5%) in the patient. For mononuclear cells, the control demonstrated 23 of 137 nuclei (17%) with one signal, versus 300 of 511 nuclei (59%) in the patient. We conclude that G-CSF induced differentiation in the dysplastic clone in this case and did not stimulate normal hermatopoiesis.
- OSTI ID:
- 133484
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0212
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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