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Title: Intein-modified enzymes, their production and industrial applications

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
1328698
Report Number(s):
9,464,333
13/508,234
DOE Contract Number:
AR0000042
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Nov 05
Research Org:
Agrivida, Inc. Medford, MA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES