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Title: The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene

Abstract

Bacterial microcompartments (MCPs) are complex organelles that consist of metabolic enzymes encapsulated within a protein shell. Here, we investigate the function of the PduJ MCP shell protein. PduJ is 80% identical in amino acid sequence to PduA and both are major shell proteins of the 1,2-propanediol (1,2-PD) utilization (Pdu) MCP of Salmonella. Prior studies showed that PduA mediates the transport of 1,2-PD (the substrate) into the Pdu MCP. Surprisingly, however, results presented here establish that PduJ has no role 1,2-PD transport. The crystal structure revealed that PduJ was nearly identical to that of PduA and, hence, offered no explanation for their differential functions. Interestingly, however, when a pduJ gene was placed at the pduA chromosomal locus, the PduJ protein acquired a new function, the ability to mediate 1,2-PD transport into the Pdu MCP. To our knowledge, these are the first studies to show that that gene location can determine the function of a MCP shell protein. We propose that gene location dictates protein-protein interactions essential to the function of the MCP shell.

Authors:
 [1];  [2];  [2];  [2];  [1]
  1. Iowa State Univ., Ames, IA (United States)
  2. Univ. of California, Los Angeles, CA (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Center for Research Resources; National Institute of General Medical Sciences (NIGMS)
OSTI Identifier:
1328044
Grant/Contract Number:  
R01AI081146; T32-GM008496; 5P41RR015301-10; 8 P41 GM103403-10; AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Molecular Microbiology
Additional Journal Information:
Journal Volume: 101; Journal Issue: 5; Journal ID: ISSN 0950-382X
Publisher:
Wiley
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chowdhury, Chiranjit, Chun, Sunny, Sawaya, Michael R., Yeates, Todd O., and Bobik, Thomas A. The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene. United States: N. p., 2016. Web. doi:10.1111/mmi.13423.
Chowdhury, Chiranjit, Chun, Sunny, Sawaya, Michael R., Yeates, Todd O., & Bobik, Thomas A. The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene. United States. https://doi.org/10.1111/mmi.13423
Chowdhury, Chiranjit, Chun, Sunny, Sawaya, Michael R., Yeates, Todd O., and Bobik, Thomas A. 2016. "The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene". United States. https://doi.org/10.1111/mmi.13423. https://www.osti.gov/servlets/purl/1328044.
@article{osti_1328044,
title = {The function of the PduJ microcompartment shell protein is determined by the genomic position of its encoding gene},
author = {Chowdhury, Chiranjit and Chun, Sunny and Sawaya, Michael R. and Yeates, Todd O. and Bobik, Thomas A.},
abstractNote = {Bacterial microcompartments (MCPs) are complex organelles that consist of metabolic enzymes encapsulated within a protein shell. Here, we investigate the function of the PduJ MCP shell protein. PduJ is 80% identical in amino acid sequence to PduA and both are major shell proteins of the 1,2-propanediol (1,2-PD) utilization (Pdu) MCP of Salmonella. Prior studies showed that PduA mediates the transport of 1,2-PD (the substrate) into the Pdu MCP. Surprisingly, however, results presented here establish that PduJ has no role 1,2-PD transport. The crystal structure revealed that PduJ was nearly identical to that of PduA and, hence, offered no explanation for their differential functions. Interestingly, however, when a pduJ gene was placed at the pduA chromosomal locus, the PduJ protein acquired a new function, the ability to mediate 1,2-PD transport into the Pdu MCP. To our knowledge, these are the first studies to show that that gene location can determine the function of a MCP shell protein. We propose that gene location dictates protein-protein interactions essential to the function of the MCP shell.},
doi = {10.1111/mmi.13423},
url = {https://www.osti.gov/biblio/1328044}, journal = {Molecular Microbiology},
issn = {0950-382X},
number = 5,
volume = 101,
place = {United States},
year = {Tue Jun 07 00:00:00 EDT 2016},
month = {Tue Jun 07 00:00:00 EDT 2016}
}

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Cited by: 23 works
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Works referenced in this record:

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