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Title: Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice

Abnormal expression of CD200/CD200R1 may contribute to the immunologic abnormalities in patients with systemic lupus erythematosus (SLE). This study aimed to assess the function of CD200/CD200R1and impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice. Female NZB/WF1 mice were treated with CD200-Fc or control for 4 weeks. Plasma samples were collected to measure autoantibody levels. The expression levels of CD200/CD200R1 in peripheral blood mononuclear cells (PBMCs) and splenocytes were examined. The percentage of CD200/CD200R1-positive cells in splenocytes from NZB/WF1 mice was lower than that of C57BL/6 mice (p<0.05). The plasma level of anti-dsDNA was significantly higher in NZB/WF1 mice than C57BL/6 mice (p<0.001). However, the anti-dsDNA levels decreased (p=0.047) after CD200-Fc treatment. Finally, CD200-Fc reduced the levels of IL-6 (p=0.017) and IL-10 (p=0.03) in the dendritic cell culture supernatant. This study suggests that the immunosuppressive CD200/CD200R1 signaling pathway might be involved in the immunopathology of NZB/WF1 mice; the present results merit further exploration of agents that can modulate the CD200/CD200FR1 pathway as a therapy for human lupus.
Authors:
 [1] ;  [1] ;  [1] ;  [1]
  1. Peking Union Medical College Hospital, Beijing (China). Key Lab. of Rheumatology & Clinical Immunology, Dept. of Rheumatology & Clinical Immunology; Chinese Academy of Medical Science (CAS), Beijing (China). Clinical Immunology Center
Publication Date:
OSTI Identifier:
1326665
Grant/Contract Number:
SC0001035; 81325019; 81172859; 81273312; 81302594; 81550023; 7141008; 20120217; 2011-4001-02
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org:
National Natural Science Foundation of China; Beijing Municipal Natural Science Foundation; USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES Lupus; CD200-Fc; NZB/WF1; Lupus nephritis