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Title: Early-branching Gut Fungi Possess A Large, And Comprehensive Array Of Biomass-Degrading Enzymes

The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. Its more primitive members, however, remain relatively unexploited. We developed a systems-level approach that integrates RNA-Seq, proteomics, phenotype and biochemical studies of relatively unexplored early-branching free-living fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, unpretreated plant biomass, and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite repressed, and are further regulated by a rich landscape of noncoding regulatory RNAs. Furthermore, we identified several promising sequence divergent enzyme candidates for lignocellulosic bioprocessing.
Authors:
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Publication Date:
OSTI Identifier:
1322507
Report Number(s):
PNNL-SA--115073
Journal ID: ISSN 0036-8075; 48839; KP1704020
DOE Contract Number:
AC05-76RL01830; AC02-05CH11231; W911NF-09-0001; SC0010352
Resource Type:
Journal Article
Resource Relation:
Journal Name: Science; Journal Volume: 351; Journal Issue: 6278
Publisher:
AAAS
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDA
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory