KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1

Wong, Sarah J.; Gearhart, Micah D.; Taylor, Alexander B.; Nanyes, David R.; Ha, Daniel J.; Robinson, Angela K.; Artigas, Jason A.; Lee, Oliver J.; Demeler, Borries; Hart, P. John; Bardwell, Vivian J.; Kim, Chongwoo A.

doi:10.1016/j.str.2016.07.011

Title: KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1

Journal Article · Sat Oct 01 00:00:00 EDT 2016 · Structure

DOI:https://doi.org/10.1016/j.str.2016.07.011· OSTI ID:1313242

Wong, Sarah J.; Gearhart, Micah D.; Taylor, Alexander B.; Nanyes, David R.; Ha, Daniel J.; Robinson, Angela K.; Artigas, Jason A.; Lee, Oliver J.; Demeler, Borries; Hart, P. John; Bardwell, Vivian J.; Kim, Chongwoo A.

KDM2B recruits H2A-ubiquitinating activity of a non-canonical Polycomb Repression Complex 1 (PRC1.1) to CpG islands, facilitating gene repression. We investigated the molecular basis of recruitment using in vitro assembly assays to identify minimal components, subcomplexes, and domains required for recruitment. A minimal four-component PRC1.1 complex can be assembled by combining two separately isolated subcomplexes: the DNA-binding KDM2B/SKP1 heterodimer and the heterodimer of BCORL1 and PCGF1, a core component of PRC1.1. The crystal structure of the KDM2B/SKP1/BCORL1/PCGF1 complex illustrates the crucial role played by the PCGF1/BCORL1 heterodimer. The BCORL1 PUFD domain positions residues preceding the RAWUL domain of PCGF1 to create an extended interface for interaction with KDM2B, which is unique to the PCGF1-containing PRC1.1 complex. The structure also suggests how KDM2B might simultaneously function in PRC1.1 and an SCF ubiquitin ligase complex and the possible molecular consequences of BCOR PUFD internal tandem duplications found in pediatric kidney and brain tumors.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Contributing Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Grant/Contract Number:: AC02–06CH11357

OSTI ID:: 1313242

Alternate ID(s):: OSTI ID: 1328788

Journal Information:: Structure, Journal Name: Structure Vol. 24 Journal Issue: 10; ISSN 0969-2126

Publisher:: ElsevierCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 32 works

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BCOR involvement in cancer Astolfi, Annalisa; Fiore, Michele; Melchionda, Fraia Epigenomics, Vol. 11, Issue 7 https://doi.org/10.2217/epi-2018-0195	journal	May 2019
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PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading Rona, Gergely; Roberti, Domenico; Yin, Yandong eLife, Vol. 7 https://doi.org/10.7554/elife.38771	journal	July 2018
Evolution and conservation of polycomb repressive complex 1 core components and putative associated factors in the green lineage Huang, Yong; Jiang, Ling; Liu, Bo-Yu BMC Genomics, Vol. 20, Issue 1 https://doi.org/10.1186/s12864-019-5905-9	journal	June 2019

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