Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human

Wang, Yaqiang; Yesselman, Joseph D.; Zhang, Qi; Kang, Mijeong; Feigon, Juli

doi:10.1073/pnas.1607411113

Title: Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human

Journal Article · Tue Aug 16 00:00:00 EDT 2016 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1607411113· OSTI ID:1297260

^[1]; Yesselman, Joseph D. ^[2]; Zhang, Qi ^[3]; Kang, Mijeong ^[4]; Feigon, Juli ^[4]

Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1569,
Biochemistry Department, Stanford University, Stanford, CA 94305,
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599,
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1569,, University of California Los Angeles–Department of Energy Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095

Telomerase is an RNA–protein complex that includes a unique reverse transcriptase that catalyzes the addition of single-stranded telomere DNA repeats onto the 3' ends of linear chromosomes using an integral telomerase RNA (TR) template. Vertebrate TR contains the template/pseudoknot (t/PK) and CR4/5 domains required for telomerase activity in vitro. All vertebrate pseudoknots include two subdomains: P2ab (helices P2a and P2b with a 5/6-nt internal loop) and the minimal pseudoknot (P2b–P3 and associated loops). A helical extension of P2a, P2a.1, is specific to mammalian TR. In this paper, using NMR, we investigated the structures of the full-length TR pseudoknot and isolated subdomains in Oryzias latipes (Japanese medaka fish), which has the smallest vertebrate TR identified to date. We determined the solution NMR structure and studied the dynamics of medaka P2ab, and identified all base pairs and tertiary interactions in the minimal pseudoknot. Despite differences in length and sequence, the structure of medaka P2ab is more similar to human P2ab than predicted, and the medaka minimal pseudoknot has the same tertiary interactions as the human pseudoknot. Significantly, although P2a.1 is not predicted to form in teleost fish, we find that it forms in the full-length pseudoknot via an unexpected hairpin. Model structures of the subdomains are combined to generate a model of t/PK. These results provide evidence that the architecture for the vertebrate t/PK is conserved from teleost fish to human. Finally, the organization of the t/PK on telomerase reverse transcriptase for medaka and human is modeled based on the cryoEM structure of Tetrahymena telomerase, providing insight into function.

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Research Organization:: Univ. of California, Los Angeles, CA (United States); Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE; National Inst. of Health (NIH) (United States); National Science Foundation (NSF)

Grant/Contract Number:: FC03-02ER63421; GM48123; GM112503; GM112294; MCB1517625

OSTI ID:: 1297260

Alternate ID(s):: OSTI ID: 1470062

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 113 Journal Issue: 35; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 21 works

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Title: Structural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to human

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