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Title: Systems and methods for the secretion of recombinant proteins in gram negative bacteria

Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
1289850
Report Number(s):
9,410,157
13/192,058
DOE Contract Number:
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Jul 27
Research Org:
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES