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Title: Leading-process actomyosin coordinates organelle positioning and adhesion receptor dynamics in radially migrating cerebellar granule neurons

During brain development, neurons migrate from germinal zones to their final positions to assemble neural circuits. A unique saltatory cadence involving cyclical organelle movement (e.g., centrosome motility) and leading-process actomyosin enrichment prior to nucleokinesis organizes neuronal migration. While functional evidence suggests that leading-process actomyosin is essential for centrosome motility, the role of the actin-enriched leading process in globally organizing organelle transport or traction forces remains unexplored. Our results show that myosin ii motors and F-actin dynamics are required for Golgi apparatus positioning before nucleokinesis in cerebellar granule neurons (CGNs) migrating along glial fibers. Moreover, we show that primary cilia are motile organelles, localized to the leading-process F-actin-rich domain and immobilized by pharmacological inhibition of myosin ii and F-actin dynamics. Finally, leading process adhesion dynamics are dependent on myosin ii and F-actin. In conclusion, we propose that actomyosin coordinates the overall polarity of migrating CGNs by controlling asymmetric organelle positioning and cell-cell contacts as these cells move along their glial guides.
 [1] ;  [1] ;  [2] ;  [1] ;  [2] ;  [1]
  1. St. Jude Children's Research Hospital, Memphis, TN (United States). Dept. of Developmental Neurobiology
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
OSTI Identifier:
Grant/Contract Number:
AC05-00OR22725; 1-FY12-455; 1R01NS066936
Accepted Manuscript
Journal Name:
Neural Development
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1749-8104
BioMed Central
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE; Work for Others (WFO); American Lebanese Syrian Associated Charities (ALSAC); National Inst. of Neurological Disorders (NINDS)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; Cerebellar granule neuron