Non-targeted Metabolomics in Diverse Sorghum Breeding Lines Indicates Primary and Secondary Metabolite Profiles Are Associated with Plant Biomass Accumulation and Photosynthesis

Turner, Marie F.; Heuberger, Adam L.; Kirkwood, Jay S.; Collins, Carl C.; Wolfrum, Edward J.; Broeckling, Corey D.; Prenni, Jessica E.; Jahn, Courtney E.

doi:10.3389/fpls.2016.00953

Title: Non-targeted Metabolomics in Diverse Sorghum Breeding Lines Indicates Primary and Secondary Metabolite Profiles Are Associated with Plant Biomass Accumulation and Photosynthesis

Journal Article · Mon Jul 11 00:00:00 EDT 2016 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2016.00953· OSTI ID:1279495

Turner, Marie F. ^[1]; Heuberger, Adam L. ^[2]; Kirkwood, Jay S. ^[3]; Collins, Carl C. ^[1]; Wolfrum, Edward J. ^[4]; Broeckling, Corey D. ^[5]; Prenni, Jessica E. ^[5]; Jahn, Courtney E. ^[1]

Colorado State Univ., Fort Collins, CO (United States). Dept. of Bioagricultural Sciences and Pest Management
Colorado State Univ., Fort Collins, CO (United States). Dept. of Horticulture and Landscape Architecture
Colorado State Univ., Fort Collins, CO (United States). Dept. of Bioagricultural Sciences and Pest Management; Colorado State Univ., Fort Collins, CO (United States). Proteomics and Metabolomics Facility
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Colorado State Univ., Fort Collins, CO (United States). Proteomics and Metabolomics Facility

Metabolomics is an emerging method to improve our understanding of how genetic diversity affects phenotypic variation in plants. Recent studies have demonstrated that genotype has a major influence on biochemical variation in several types of plant tissues, however, the association between metabolic variation and variation in morphological and physiological traits is largely unknown. Sorghum bicolor (L.) is an important food and fuel crop with extensive genetic and phenotypic variation. Sorghum lines have been bred for differing phenotypes beneficial for production of grain (food), stem sugar (food, fuel), and cellulosic biomass (forage, fuel), and these varying phenotypes are the end products of innate metabolic programming which determines how carbon is allocated during plant growth and development. Further, sorghum has been adapted among highly diverse environments. Because of this geographic and phenotypic variation, the sorghum metabolome is expected to be highly divergent; however, metabolite variation in sorghum has not been characterized. Here, we utilize a phenotypically diverse panel of sorghum breeding lines to identify associations between leaf metabolites and morpho-physiological traits. The panel (11 lines) exhibited significant variation for 21 morpho-physiological traits, as well as broader trends in variation by sorghum type (grain vs. biomass types). Variation was also observed for cell wall constituents (glucan, xylan, lignin, ash). Non-targeted metabolomics analysis of leaf tissue showed that 956 of 1181 metabolites varied among the lines (81%, ANOVA, FDR adjusted p < 0.05). Both univariate and multivariate analyses determined relationships between metabolites and morpho-physiological traits, and 384 metabolites correlated with at least one trait (32%, p < 0.05), including many secondary metabolites such as glycosylated flavonoids and chlorogenic acids. The use of metabolomics to explain relationships between two or more morpho-physiological traits was explored and showed chlorogenic and shikimic acid to be associated with photosynthesis, early plant growth and final biomass measures in sorghum. In conclusion, taken together, this study demonstrates the integration of metabolomics with morpho-physiological datasets to elucidate links between plant metabolism, growth, and architecture.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1279495

Report Number(s):: NREL/JA-5100-66879

Journal Information:: Frontiers in Plant Science, Vol. 7; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 59 works

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Translational Metabolomics: Current Challenges and Future Opportunities Pinu, Farhana R.; Goldansaz, Seyed Ali; Jaine, Jacob Metabolites, Vol. 9, Issue 6 https://doi.org/10.3390/metabo9060108	journal	June 2019
Metabolite profiling and genome-wide association studies reveal response mechanisms of phosphorus deficiency in maize seedling Luo, Bowen; Ma, Peng; Nie, Zhi The Plant Journal, Vol. 97, Issue 5 https://doi.org/10.1111/tpj.14160	journal	January 2019
Distinguishing Six Edible Berries Based on Metabolic Pathway and Bioactivity Correlations by Non-targeted Metabolite Profiling Suh, Dong Ho; Jung, Eun Sung; Lee, Gyu Min Frontiers in Plant Science, Vol. 9 https://doi.org/10.3389/fpls.2018.01462	journal	October 2018
Non-Targeted Metabolomics Reveals Sorghum Rhizosphere-Associated Exudates are Influenced by the Belowground Interaction of Substrate and Sorghum Genotype Miller, Sarah B.; Heuberger, Adam L.; Broeckling, Corey D. International Journal of Molecular Sciences, Vol. 20, Issue 2 https://doi.org/10.3390/ijms20020431	journal	January 2019

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