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This content will become publicly available on June 24, 2017

Title: Recent Advances in Algal Genetic Tool Development

The goal of achieving cost-effective biofuels and bioproducts derived from algal biomass will require improvements along the entire value chain, including identification of robust, high-productivity strains and development of advanced genetic tools. Though there have been modest advances in development of genetic systems for the model alga Chlamydomonas reinhardtii, progress in development of algal genetic tools, especially as applied to non-model algae, has generally lagged behind that of more commonly utilized laboratory and industrial microbes. This is in part due to the complex organellar structure of algae, including robust cell walls and intricate compartmentalization of target loci, as well as prevalent gene silencing mechanisms, which hinder facile utilization of conventional genetic engineering tools and methodologies. However, recent progress in global tool development has opened the door for implementation of strain-engineering strategies in industrially-relevant algal strains. Here, we review recent advances in algal genetic tool development and applications in eukaryotic microalgae.
Authors:
 [1] ;  [2]
  1. Colorado School of Mines, Golden, CO (United States). Dept. of Chemistry and Geochemistry
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Publication Date:
OSTI Identifier:
1271943
Report Number(s):
NREL/JA-5100-65870
Journal ID: ISSN 2211-5501
Grant/Contract Number:
AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Current Biotechnology
Additional Journal Information:
Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2211-5501
Publisher:
Bentham Science Publishers
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; algae; genetic engineering; biofuels; bioproducts; synthetic biology; photosynthesis