Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina
Abstract
Productivity of algal mass culture can be severely reduced by contaminating organisms. It is, therefore, important to identify contaminants, determine their effect on productivity and, ultimately, develop countermeasures against such contamination. In this paper, we utilized microbiome analysis by second-generation sequencing of small subunit rRNA genes to characterize the predator and pathogen burden of open raceway cultures of Nannochloropsis salina. Samples were analyzed from replicate raceways before and after crashes. In one culture cycle, we identified two algivorous species, the rotifer Brachionus and gastrotrich Chaetonotus, the presence of which may have contributed to the loss of algal biomass. In the second culture cycle, the raceways were treated with hypochlorite in an unsuccessful attempt to interdict the crash. Finally, our analyses were shown to be an effective strategy for the identification of the biological contaminants and the characterization of intervention strategies.
- Authors:
-
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Biology
- Texas A&M AgriLife Research and Extension Center, Corpus Christi, TX (United States). AgriLife Research Mariculture Lab. at Flour Bluff
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- Contributing Org.:
- Texas A&M AgriLife Research and Extension Center, Corpus Christi, TX (United States)
- OSTI Identifier:
- 1271304
- Alternate Identifier(s):
- OSTI ID: 1323980
- Report Number(s):
- SAND2015-5591J
Journal ID: ISSN 2211-9264; PII: S2211926416301679
- Grant/Contract Number:
- AC04-94AL85000; NL0022897
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Algal Research
- Additional Journal Information:
- Journal Volume: 17; Journal ID: ISSN 2211-9264
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Algae mass culture; Biocontamination; Biomass algal crash; Second generation sequencing; Microbiome analysis
Citation Formats
Carney, Laura T., Wilkenfeld, Joshua S., Lane, Pam D., Solberg, Owen D., Fuqua, Zachary B., Cornelius, Nina G., Gillespie, Shaunette, Williams, Kelly P., Samocha, Tzachi M., and Lane, Todd W. Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina. United States: N. p., 2016.
Web. doi:10.1016/j.algal.2016.05.011.
Carney, Laura T., Wilkenfeld, Joshua S., Lane, Pam D., Solberg, Owen D., Fuqua, Zachary B., Cornelius, Nina G., Gillespie, Shaunette, Williams, Kelly P., Samocha, Tzachi M., & Lane, Todd W. Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina. United States. https://doi.org/10.1016/j.algal.2016.05.011
Carney, Laura T., Wilkenfeld, Joshua S., Lane, Pam D., Solberg, Owen D., Fuqua, Zachary B., Cornelius, Nina G., Gillespie, Shaunette, Williams, Kelly P., Samocha, Tzachi M., and Lane, Todd W. 2016.
"Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina". United States. https://doi.org/10.1016/j.algal.2016.05.011. https://www.osti.gov/servlets/purl/1271304.
@article{osti_1271304,
title = {Pond Crash Forensics: Presumptive identification of pond crash agents by next generation sequencing in replicate raceway mass cultures of Nannochloropsis salina},
author = {Carney, Laura T. and Wilkenfeld, Joshua S. and Lane, Pam D. and Solberg, Owen D. and Fuqua, Zachary B. and Cornelius, Nina G. and Gillespie, Shaunette and Williams, Kelly P. and Samocha, Tzachi M. and Lane, Todd W.},
abstractNote = {Productivity of algal mass culture can be severely reduced by contaminating organisms. It is, therefore, important to identify contaminants, determine their effect on productivity and, ultimately, develop countermeasures against such contamination. In this paper, we utilized microbiome analysis by second-generation sequencing of small subunit rRNA genes to characterize the predator and pathogen burden of open raceway cultures of Nannochloropsis salina. Samples were analyzed from replicate raceways before and after crashes. In one culture cycle, we identified two algivorous species, the rotifer Brachionus and gastrotrich Chaetonotus, the presence of which may have contributed to the loss of algal biomass. In the second culture cycle, the raceways were treated with hypochlorite in an unsuccessful attempt to interdict the crash. Finally, our analyses were shown to be an effective strategy for the identification of the biological contaminants and the characterization of intervention strategies.},
doi = {10.1016/j.algal.2016.05.011},
url = {https://www.osti.gov/biblio/1271304},
journal = {Algal Research},
issn = {2211-9264},
number = ,
volume = 17,
place = {United States},
year = {Thu Jun 02 00:00:00 EDT 2016},
month = {Thu Jun 02 00:00:00 EDT 2016}
}
Web of Science
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