A transgenic approach for controlling Lygus in cotton

Gowda, Anilkumar; Rydel, Timothy J.; Wollacott, Andrew M.; Brown, Robert S.; Akbar, Waseem; Clark, Thomas L.; Flasinski, Stanislaw; Nageotte, Jeffrey R.; Read, Andrew C.; Shi, Xiaohong; Werner, Brent J.; Pleau, Michael J.; Baum, James A.

doi:10.1038/ncomms12213

Title: A transgenic approach for controlling Lygus in cotton

Journal Article · Mon Jul 18 00:00:00 EDT 2016 · Nature Communications

DOI:https://doi.org/10.1038/ncomms12213· OSTI ID:1267458

Gowda, Anilkumar ^[1]; Rydel, Timothy J. ^[1]; Wollacott, Andrew M. ^[2]; Brown, Robert S. ^[1]; Akbar, Waseem ^[1]; Clark, Thomas L. ^[1]; Flasinski, Stanislaw ^[1]; Nageotte, Jeffrey R. ^[1]; Read, Andrew C. ^[3]; Shi, Xiaohong ^[1]; Werner, Brent J. ^[1]; Pleau, Michael J. ^[1]; Baum, James A. ^[1]

Monsanto Company, Chesterfield, MO (United States)
Monsanto Company, Chesterfield, MO (United States); Visterra Inc, Cambridge, MA (United States)
Cornell Univ., Ithaca, NY (United States); Monsanto Company, Chesterfield, MO (United States)

Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

OSTI ID:: 1267458

Journal Information:: Nature Communications, Vol. 7, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 50 works

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First transgenic trait for control of plant bugs and thrips in cotton: Bt cotton controls plant bugs and thrips Akbar, Waseem; Gowda, Anilkumar; Ahrens, Jeffrey E. Pest Management Science, Vol. 75, Issue 3 https://doi.org/10.1002/ps.5234	journal	December 2018
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Transgenic crops for the agricultural improvement in Pakistan: a perspective of environmental stresses and the current status of genetically modified crops Babar, Usman; Nawaz, Muhammad Amjad; Arshad, Usama GM Crops & Food https://doi.org/10.1080/21645698.2019.1680078	journal	November 2019
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