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Title: Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park

Abstract

Geobacillus thermoglucosidasius Y4.1MC1 was isolated from a boiling spring in the lower geyser basin of Yellowstone National Park. We present this species is of interest because of its metabolic versatility. The genome consists of one circular chromosome of 3,840,330 bp and a circular plasmid of 71,617 bp with an average GC content of 44.01%. The genome is available in the GenBank database (NC_014650.1 and NC_014651.1). In addition to the expected metabolic pathways for sugars and amino acids, the Y4.1MC1 genome codes for two separate carbon monoxide utilization pathways, an aerobic oxidation pathway and an anaerobic reductive acetyl CoA (Wood-Ljungdahl) pathway. This is the first report of a nonanaerobic organism with the Wood-Ljungdahl pathway. Also, this anaerobic pathway permits the strain to utilize H2 and fix CO2 present in the hot spring environment. Y4.1MC1 and its related species may play a significant role in carbon capture and sequestration in thermophilic ecosystems and may open up new routes to produce biofuels and chemicals from CO, H2, and CO2.

Authors:
 [1];  [2];  [2];  [3];  [3];  [4]
  1. C5.6 Technologies Inc., Middleton, WI (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division.
  4. Lucigen Corporation, Middleton, WI (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Work for Others (WFO)
OSTI Identifier:
1265371
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 8; Journal Issue: 3; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Carbon monoxide . Carbon fixation . Wood-Ljungdahl pathway . YellowstoneNational Park . Geobacillus thermoglucosidasius

Citation Formats

Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A. Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9585-2.
Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, & Mead, David A. Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park. United States. https://doi.org/10.1007/s12155-015-9585-2
Brumm, Phillip, Land, Miriam L., Hauser, Loren John, Jeffries, Cynthia D., Chang, Yun-Juan, and Mead, David A. 2015. "Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park". United States. https://doi.org/10.1007/s12155-015-9585-2. https://www.osti.gov/servlets/purl/1265371.
@article{osti_1265371,
title = {Complete genome sequence of Geobacillus strain Y4.1MC1, a novel CO-utilizing Geobacillus thermoglucosidasius strain isolated from Bath Hot Spring in Yellowstone National Park},
author = {Brumm, Phillip and Land, Miriam L. and Hauser, Loren John and Jeffries, Cynthia D. and Chang, Yun-Juan and Mead, David A.},
abstractNote = {Geobacillus thermoglucosidasius Y4.1MC1 was isolated from a boiling spring in the lower geyser basin of Yellowstone National Park. We present this species is of interest because of its metabolic versatility. The genome consists of one circular chromosome of 3,840,330 bp and a circular plasmid of 71,617 bp with an average GC content of 44.01%. The genome is available in the GenBank database (NC_014650.1 and NC_014651.1). In addition to the expected metabolic pathways for sugars and amino acids, the Y4.1MC1 genome codes for two separate carbon monoxide utilization pathways, an aerobic oxidation pathway and an anaerobic reductive acetyl CoA (Wood-Ljungdahl) pathway. This is the first report of a nonanaerobic organism with the Wood-Ljungdahl pathway. Also, this anaerobic pathway permits the strain to utilize H2 and fix CO2 present in the hot spring environment. Y4.1MC1 and its related species may play a significant role in carbon capture and sequestration in thermophilic ecosystems and may open up new routes to produce biofuels and chemicals from CO, H2, and CO2.},
doi = {10.1007/s12155-015-9585-2},
url = {https://www.osti.gov/biblio/1265371}, journal = {BioEnergy Research},
issn = {1939-1234},
number = 3,
volume = 8,
place = {United States},
year = {Tue Feb 10 00:00:00 EST 2015},
month = {Tue Feb 10 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 15 works
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Figures / Tables:

Table 1 Table 1: Summary of genome

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Works referenced in this record:

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journal, March 1998


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journal, January 2007


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journal, May 2010


Protein-based organelles in bacteria: carboxysomes and related microcompartments
journal, August 2008


Distribution and molecular investigation of highly thermophilic bacteria associated with cool soil environments
journal, April 2004


RNAmmer: consistent and rapid annotation of ribosomal RNA genes
journal, April 2007


Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park
journal, November 2004

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journal, March 1998


Analysis of the gluconate (gnt) operon of Bacillus subtilis
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Works referencing / citing this record:

Unraveling the cellulolytic and hemicellulolytic potential of two novel Streptomyces strains
journal, September 2018


Geobacillus yumthangensis sp. nov., a thermophilic bacterium isolated from a north-east Indian hot spring
journal, November 2018


Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment
journal, January 2019


Comparative genomic analysis of Parageobacillus thermoglucosidasius strains with distinct hydrogenogenic capacities
journal, December 2018


Complete genome sequences of Geobacillus sp. WCH70, a thermophilic strain isolated from wood compost
journal, April 2016


Draft Genome Sequence of Parageobacillus thermoglucosidasius Strain TG4, a Hydrogenogenic Carboxydotrophic Bacterium Isolated from a Marine Sediment
journal, January 2019


Comparative genomic analysis of Parageobacillus thermoglucosidasius strains with distinct hydrogenogenic capacities
journal, December 2018


Complete genome sequences of Geobacillus sp. WCH70, a thermophilic strain isolated from wood compost
journal, April 2016


Complete Genome Sequence of Thermus aquaticus Y51MC23
journal, October 2015


Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.