Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

Rodriguez, Jose A.; Xu, Rui; Chen, Chien -Chun; Huang, Zhifeng; Jiang, Huaidong; Chen, Allan L.; Raines, Kevin S.; Pryor, Jr., Alan; Nam, Daewoong; Wiegart, Lutz; Song, Changyong; Madsen, Anders; Chushkin, Yuriy; Zontone, Federico; Bradley, Peter J.; Miao, Jianwei

doi:10.1107/s205225251501235x

Title: Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

Journal Article · Tue Sep 01 00:00:00 EDT 2015 · IUCrJ

DOI:https://doi.org/10.1107/s205225251501235x· OSTI ID:1261644

Rodriguez, Jose A. ^[1]; Xu, Rui ^[1]; Chen, Chien -Chun ^[2]; Huang, Zhifeng ^[3]; Jiang, Huaidong ^[4]; Chen, Allan L. ^[1]; Raines, Kevin S. ^[5]; Pryor, Jr., Alan ^[1]; Nam, Daewoong ^[6]; Wiegart, Lutz ^[7]; Song, Changyong ^[6]; Madsen, Anders ^[8]; Chushkin, Yuriy ^[9]; Zontone, Federico ^[9]; Bradley, Peter J. ^[1]; Miao, Jianwei ^[1]

Univ. of California, Los Angeles, CA (United States)
National Sun Yat-sen Univ., Kaohsiung (Taiwan)
Carl ZEISS X-ray Microscopy, Inc., Pleasanton, CA (United States)
Shandong Univ., Jinan (People's Republic of China)
Stanford Univ., Stanford, CA (United States)
Pohang Univ. of Science and Technology, Pohang (South Korea)
Brookhaven National Lab. (BNL), Upton, NY (United States)
European X-ray Free Electron Laser, Hamburg (Germany)
ESRF, Grenoble (France)

Here, a structural understanding of whole cells in three dimensions at high spatial resolution remains a significant challenge and, in the case of X-rays, has been limited by radiation damage. By alleviating this limitation, cryogenic coherent diffractive imaging (cryo-CDI) can in principle be used to bridge the important resolution gap between optical and electron microscopy in bio-imaging. Here, the first experimental demonstration of cryo-CDI for quantitative three-dimensional imaging of whole frozen-hydrated cells using 8 Kev X-rays is reported. As a proof of principle, a tilt series of 72 diffraction patterns was collected from a frozen-hydrated Neospora caninum cell and the three-dimensional mass density of the cell was reconstructed and quantified based on its natural contrast. This three-dimensional reconstruction reveals the surface and internal morphology of the cell, including its complex polarized sub-cellular structure. Finally, it is believed that this work represents an experimental milestone towards routine quantitative three-dimensional imaging of whole cells in their natural state with spatial resolutions in the tens of nanometres.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE; Defense Advanced Research Projects Agency (DARPA); National Institutes of Health (NIH); National Natural Science Foundation of China (NSFC)

Grant/Contract Number:: DARPA-BAA-12-63; GM081409- 01A1; R01#AI064616; 31430031

OSTI ID:: 1261644

Journal Information:: IUCrJ, Vol. 2, Issue 5; ISSN 2052-2525

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 49 works

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62 RADIOLOGY AND NUCLEAR MEDICINE
coherent diffractive imaging
cryo-CDI
three-dimensional imaging
three-dimensional cellular structure
coherent diffraction
X-ray imaging
Neospora caninum

Title: Three-dimensional coherent X-ray diffractive imaging of whole frozen-hydrated cells

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