Programming A Molecular Relay for Ultrasensitive Biodetection through 129 Xe NMR

Wang, Yanfei; Roose, Benjamin W.; Philbin, John P.; Doman, Jordan L.; Dmochowski, Ivan J.

doi:10.1002/anie.201508990

Title: Programming A Molecular Relay for Ultrasensitive Biodetection through ¹²⁹ Xe NMR

Journal Article · Mon Dec 21 00:00:00 EST 2015 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.201508990· OSTI ID:1260842

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^[1]; Philbin, John P. ^[1]; Doman, Jordan L. ^[1];

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Univ. of Pennsylvania, Philadelphia, PA (United States)

We reported a supramolecular strategy for detecting specific proteins in complex media by using hyperpolarized ¹²⁹Xe NMR. A cucurbit[6]uril (CB[6])-based molecular relay was programmed for three sequential equilibrium conditions by designing a two-faced guest (TFG) that initially binds CB[6] and blocks the CB[6]–Xe interaction. Moreover, the protein analyte recruits the TFG and frees CB[6] for Xe binding. TFGs containing CB[6]- and carbonic anhydrase II (CAII)-binding domains were synthesized in one or two steps. X-ray crystallography confirmed TFG binding to Zn²⁺ in the deep CAII active-site cleft, which precludes simultaneous CB[6] binding. The molecular relay was reprogrammed to detect avidin by using a different TFG. Finally, Xe binding by CB[6] was detected in buffer and in E. coli cultures expressing CAII through ultrasensitive ¹²⁹Xe NMR spectroscopy.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: R01-GM097478; LC130824; S10-DO016260

OSTI ID:: 1260842

Journal Information:: Angewandte Chemie (International Edition), Vol. 55, Issue 5; ISSN 1433-7851

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 23 works

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
bioinorganic chemistry
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Title: Programming A Molecular Relay for Ultrasensitive Biodetection through 129 Xe NMR

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Title: Programming A Molecular Relay for Ultrasensitive Biodetection through ¹²⁹ Xe NMR