Antibody-based biological toxin detection
Abstract
Fiber optic evanescent fluorosensors are under investigation in our laboratory for the study of drug-receptor interactions for detection of threat agents and antibody-antigen interactions for detection of biological toxins. In a direct competition assay, antibodies against Cholera toxin, Staphylococcus Enterotoxin B or ricin were noncovalently immobilized on quartz fibers and probed with fluorescein isothiocyanate (FITC) - labeled toxins. In the indirect competition assay, Cholera toxin or Botulinum toxoid A was immobilized onto the fiber, followed by incubation in an antiserum or partially purified anti-toxin IgG. These were then probed with FITC-anti-IgG antibodies. Unlabeled toxins competed with labeled toxins or anti-toxin IgG in a dose dependent manner and the detection of the toxins was in the nanomolar range.
- Authors:
-
- Army Edgewood Research, Development and Engineering Center, Aberdeen Proving Ground, MD (United States)
- Publication Date:
- OSTI Identifier:
- 126042
- Report Number(s):
- CONF-950402-
TRN: 95:006086-0010
- Resource Type:
- Conference
- Resource Relation:
- Conference: 209. American Chemical Society (ACS) national meeting, Anaheim, CA (United States), 2-6 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of 209th ACS national meeting; PB: 2088 p.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; 44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; STAPHYLOCOCCUS; DETECTION; TOXINS; CHOLERA; ANTIBODIES; FLUORESCEIN; BIOLOGICAL WARFARE AGENTS; ISOTHIOCYANATES; LABELLED COMPOUNDS; FIBER OPTICS
Citation Formats
Menking, D E, and Goode, M T. Antibody-based biological toxin detection. United States: N. p., 1995.
Web.
Menking, D E, & Goode, M T. Antibody-based biological toxin detection. United States.
Menking, D E, and Goode, M T. 1995.
"Antibody-based biological toxin detection". United States.
@article{osti_126042,
title = {Antibody-based biological toxin detection},
author = {Menking, D E and Goode, M T},
abstractNote = {Fiber optic evanescent fluorosensors are under investigation in our laboratory for the study of drug-receptor interactions for detection of threat agents and antibody-antigen interactions for detection of biological toxins. In a direct competition assay, antibodies against Cholera toxin, Staphylococcus Enterotoxin B or ricin were noncovalently immobilized on quartz fibers and probed with fluorescein isothiocyanate (FITC) - labeled toxins. In the indirect competition assay, Cholera toxin or Botulinum toxoid A was immobilized onto the fiber, followed by incubation in an antiserum or partially purified anti-toxin IgG. These were then probed with FITC-anti-IgG antibodies. Unlabeled toxins competed with labeled toxins or anti-toxin IgG in a dose dependent manner and the detection of the toxins was in the nanomolar range.},
doi = {},
url = {https://www.osti.gov/biblio/126042},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Dec 01 00:00:00 EST 1995},
month = {Fri Dec 01 00:00:00 EST 1995}
}