Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus

Gardner, Jeffrey G.

doi:10.1007/s11274-016-2068-6

Title: Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus

Abstract

Study of recalcitrant polysaccharide degradation by bacterial systems is critical for understanding biological processes such as global carbon cycling, nutritional contributions of the human gut microbiome, and the production of renewable fuels and chemicals. One bacterium that has a robust ability to degrade polysaccharides is the Gram-negative saprophyte Cellvibrio japonicus. A bacterium with a circuitous history, C. japonicus underwent several taxonomy changes from an initially described Pseudomonas sp. Most of the enzymes described in the pre-genomics era have also been renamed. Furthermore, this review aims to consolidate the biochemical, structural, and genetic data published on C. japonicus and its remarkable ability to degrade cellulose, xylan, and pectin substrates. Initially, C. japonicus carbohydrate-active enzymes were studied biochemically and structurally for their novel polysaccharide binding and degradation characteristics, while more recent systems biology approaches have begun to unravel the complex regulation required for lignocellulose degradation in an environmental context. Also included is a discussion for the future of C. japonicus as a model system, with emphasis on current areas unexplored in terms of polysaccharide degradation and emerging directions for C. japonicus in both environmental and biotechnological applications.

Authors:

Gardner, Jeffrey G. ^[1]

Univ. of Maryland, College Park, MD (United States)

Publication Date:: Sat Jun 04 00:00:00 EDT 2016

Research Org.:: Univ. of Maryland, College Park, MD (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1259846

Grant/Contract Number:: SC0014183

Resource Type:: Journal Article: Accepted Manuscript

Journal Name:: World Journal of Microbiology and Biotechnology

Additional Journal Information:: Journal Volume: 32; Journal Issue: 7; Journal ID: ISSN 0959-3993

Publisher:: Springer

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; carbohydrate active enzyme; Cellvibrio japonicus; lignocellulose; polysaccharide; degradation; saprophyte

Citation Formats


                    Gardner, Jeffrey G. Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus.  United States: N. p., 2016. 
        Web.  doi:10.1007/s11274-016-2068-6.

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                    Gardner, Jeffrey G. Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus.  United States.  https://doi.org/10.1007/s11274-016-2068-6

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                    Gardner, Jeffrey G. 2016.  
        "Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus".  United States.  https://doi.org/10.1007/s11274-016-2068-6.  https://www.osti.gov/servlets/purl/1259846.

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@article{osti_1259846,

  title        = {Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus},

  author       = {Gardner, Jeffrey G.},

  abstractNote = {Study of recalcitrant polysaccharide degradation by bacterial systems is critical for understanding biological processes such as global carbon cycling, nutritional contributions of the human gut microbiome, and the production of renewable fuels and chemicals. One bacterium that has a robust ability to degrade polysaccharides is the Gram-negative saprophyte Cellvibrio japonicus. A bacterium with a circuitous history, C. japonicus underwent several taxonomy changes from an initially described Pseudomonas sp. Most of the enzymes described in the pre-genomics era have also been renamed. Furthermore, this review aims to consolidate the biochemical, structural, and genetic data published on C. japonicus and its remarkable ability to degrade cellulose, xylan, and pectin substrates. Initially, C. japonicus carbohydrate-active enzymes were studied biochemically and structurally for their novel polysaccharide binding and degradation characteristics, while more recent systems biology approaches have begun to unravel the complex regulation required for lignocellulose degradation in an environmental context. Also included is a discussion for the future of C. japonicus as a model system, with emphasis on current areas unexplored in terms of polysaccharide degradation and emerging directions for C. japonicus in both environmental and biotechnological applications.},

  doi          = {10.1007/s11274-016-2068-6},

  url          = {https://www.osti.gov/biblio/1259846},
  journal      = {World Journal of Microbiology and Biotechnology},

  issn         = {0959-3993},

  number       = 7,

  volume       = 32,

  place        = {United States},

  year         = {Sat Jun 04 00:00:00 EDT 2016},

  month        = {Sat Jun 04 00:00:00 EDT 2016}

}

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https://doi.org/10.1128/MMBR.66.3.506-577.2002

Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase
journal, September 2001

Beylot, Marie-Helene; Emami, Kaveh; McKIE, Vincent A.
Biochemical Journal, Vol. 358, Issue 3
https://doi.org/10.1042/bj3580599

The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre
journal, May 1990

Gilbert, H. J.; Hall, J.; Hazlewood, G. P.
Molecular Microbiology, Vol. 4, Issue 5
https://doi.org/10.1111/j.1365-2958.1990.tb00646.x

Structure of a Mannan-specific Family 35 Carbohydrate-Binding Module: Evidence for Significant Conformational Changes upon Ligand Binding
journal, March 2005

Tunnicliffe, Richard B.; Bolam, David N.; Pell, Gavin
Journal of Molecular Biology, Vol. 347, Issue 2
https://doi.org/10.1016/j.jmb.2005.01.038

Carbohydrate-binding modules: fine-tuning polysaccharide recognition
journal, September 2004

Boraston, Alisdair B.; Bolam, David N.; Gilbert, Harry J.
Biochemical Journal, Vol. 382, Issue 3
https://doi.org/10.1042/BJ20040892

Cellvibrio japonicus α-L-arabinanase 43A has a novel five-blade β-propeller fold
journal, August 2002

Nurizzo, Didier; Turkenburg, Johan P.; Charnock, Simon J.
Nature Structural Biology, Vol. 9, Issue 9
https://doi.org/10.1038/nsb835

Regulation of the Xylan-degrading Apparatus of Cellvibrio japonicus by a Novel Two-component System
journal, October 2008

Emami, Kaveh; Topakas, Evangelos; Nagy, Tibor
Journal of Biological Chemistry, Vol. 284, Issue 2
https://doi.org/10.1074/jbc.M805100200

Dividing the Large Glycoside Hydrolase Family 43 into Subfamilies: a Motivation for Detailed Enzyme Characterization
journal, January 2016

Mewis, Keith; Lenfant, Nicolas; Lombard, Vincent
Applied and Environmental Microbiology, Vol. 82, Issue 6
https://doi.org/10.1128/AEM.03453-15

Requirement of the Type II Secretion System for Utilization of Cellulosic Substrates by Cellvibrio japonicus
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Gardner, J. G.; Keating, D. H.
Applied and Environmental Microbiology, Vol. 76, Issue 15
https://doi.org/10.1128/AEM.00454-10

NMR Spectroscopic Analysis Reveals Extensive Binding Interactions of Complex Xyloglucan Oligosaccharides with the Cellvibrio japonicus Glycoside Hydrolase Family 31 α-Xylosidase
journal, September 2012

Silipo, Alba; Larsbrink, Johan; Marchetti, Roberta
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Inactivated enzymes as probes of the structure of arabinoxylans as observed by atomic force microscopy
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Adams, Elizabeth L.; Kroon, Paul A.; Williamson, Gary
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Yamane, Kunio; Yoshikawa, Takafumi; Suzuki, Hiroshi
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Hall, J.; Hazlewood, G. P.; Huskisson, N. S.
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Tailored catalysts for plant cell-wall degradation: Redesigning the exo/endo preference of Cellvibrio japonicus arabinanase 43A
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Proctor, M. R.; Taylor, E. J.; Nurizzo, D.
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Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes
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Levasseur, Anthony; Drula, Elodie; Lombard, Vincent
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An Aspergillus niger esterase (ferulic acid esterase III) and a recombinant Pseudomonas fluorescens subsp. cellulosa esterase (Xy1D) release a 5-5' ferulic dehydrodimer (diferulic acid) from barley and wheat cell walls.
journal, January 1997

Bartolomé, B.; Faulds, C. B.; Kroon, P. A.
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Characterization of an Endoglucanase from Pseudomonas fluorescens subsp. cellulosa Produced in Escherichia coli and Regulation of the Expression of Its Cloned Gene
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Lejeune, André; Courtois, Stéphane; Colson, Charles
Applied and Environmental Microbiology, Vol. 54, Issue 2
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Isolation of Endoglucanase Genes from Pseudomonas fluorescens subsp. cellulosa and a Pseudomonas sp
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Wolff, Bruce R.; Mudry, Terry A.; Glick, Bernard R.
Applied and Environmental Microbiology, Vol. 51, Issue 6
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Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module
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E. Brown, Ian; H. Mallen, Marie; J. Charnock, Simon
Biochemical Journal, Vol. 355, Issue 1
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The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity
journal, September 2001

Beylot, Marie-Helene; McKIE, Vincent A.; Voragen, Alphons G. J.
Biochemical Journal, Vol. 358, Issue 3
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The resistance of cellulases and xylanases to proteolytic inactivation
journal, April 1995

Fontes, C. M. G. A.; Hall, J.; Hirst, B. H.
Applied Microbiology and Biotechnology, Vol. 43, Issue 1
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The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism
journal, September 1999

Gill, Jaitinder; Rixon, Jane E.; Bolam, David N.
Biochemical Journal, Vol. 342, Issue 2
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A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose
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McKIE, V. A.; Vincken, J. -P.; Voragen, A. G. J.
Biochemical Journal, Vol. 365, Issue 3
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Hazlewood, G. P.; Gilbert, H. J.
Biochemical Society Transactions, Vol. 26, Issue 2
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A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose
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A. McKIE, Vincent; Vincken, Jean-Paul; G. J. Voragen, Alphons
Biochemical Journal, Vol. 355, Issue 1
https://doi.org/10.1042/0264-6021:3550167

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Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy
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The complex physiology of Cellvibrio japonicus xylan degradation relies on a single cytoplasmic β-xylosidase for xylo-oligosaccharide utilization

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Summary Lignocellulose degradation by microbes plays a central role in global carbon cycling, human gut metabolism and renewable energy technologies. While considerable effort has been put into understanding the biochemical aspects of lignocellulose degradation, much less work has been done to understand how these enzymes work in an in vivo context. Here, we report a systems level study of xylan degradation in the saprophytic bacterium Cellvibrio japonicus . Transcriptome analysis indicated seven genes that encode carbohydrate active enzymes were up‐regulated during growth with xylan containing media. In‐frame deletion analysis of these genes found that only gly43F is critical for utilizationmore »« less
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Title: Polysaccharide degradation systems of the saprophytic bacterium Cellvibrio japonicus

Abstract

Citation Formats

The Structure and Function of an Arabinan-specific α-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases journal, February 2011

A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions The GenBank accession numbers for the sequences described in this paper are AF049493 and AF168359 for xynC and xynG, respectively. journal, August 2000

The modular architecture of Cellvibrio japonicus mannanases in glycoside hydrolase families 5 and 26 points to differences in their role in mannan degradation journal, May 2003

The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism journal, August 1999

Crystallization and preliminary X-ray diffraction studies of a family 26 endo-β-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa journal, January 1998

DNA sequence analysis of endoglucanase genes fromPseudomonas fluorescens subsp.cellulosa andPseudomonas sp. NCIB 8634 journal, December 1990

The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases: The Production Of Ca2+-Independent Stable Xylanases journal, September 2004

Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase journal, July 1990

Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa journal, February 1999

The resistance of cellulases and xylanases to proteolytic inactivation journal, April 1995

The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains journal, November 1991

A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain journal, September 1993

Biogenesis of Multiple Cellulase Components of Pseudomonas fluorescens var. cellulosa journal, March 1974

The Structural Basis for Catalysis and Specificity of the Pseudomonas cellulosa α-Glucuronidase, GlcA67A journal, April 2002

Refined Crystal Structure of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens at 1.8 Å Resolution journal, March 1996

The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions journal, March 2009

The α-Glucuronidase, GlcA67A, of Cellvibrio japonicus Utilizes the Carboxylate and Methyl Groups of Aldobiouronic Acid as Important Substrate Recognition Determinants journal, March 2003

Novel cellulose-binding domains, NodB homologues and conserved modular architecture in xylanases from the aerobic soil bacteria Pseudomonas fluorescens subsp. cellulosa and Cellvibrio mixtus journal, November 1995

Mannanase A from Pseudomonas fluorescens ssp. cellulosa Is a Retaining Glycosyl Hydrolase in Which E212 and E320 Are the Putative Catalytic Residues † journal, January 1996

The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics journal, January 2009

Characterization of the cellulose-degrading bacterium NCIMB 10462 journal, September 1995

A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose journal, February 2001

The molecular biology of chitin digestion journal, June 1998

Evidence for multiple carboxymethylcellulase genes in Pseudomonas fluorescens subsp. cellulosa journal, January 1987

Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites journal, November 1994

Starch turnover: pathways, regulation and role in growth journal, June 2012

Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature journal, January 2015

The carbohydrate-active enzymes database (CAZy) in 2013 journal, November 2013

Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus journal, February 2016

Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module journal, February 2001

The Cellvibrio japonicus Mannanase Cj Man26C Displays a Unique exo -Mode of Action That Is Conferred by Subtle Changes to the Distal Region of the Active Site journal, September 2008

Expression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis journal, March 1988

Insights into Plant Cell Wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus journal, June 2008

Lytic Polysaccharide Monooxygenases in Biomass Conversion journal, December 2015

A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes journal, January 2014

Characterization of the gene celD and its encoded product 1,4-β-d-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa journal, August 1992

Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo- mode of action journal, April 1997

Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11 journal, August 2002

Pseudomonas fluorescens subsp. cellulosa : an alternative model for bacterial cellulase journal, March 1992

Reclassification of 'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. journal, March 2003

Importance of Hydrophobic and Polar Residues in Ligand Binding in the Family 15 Carbohydrate-Binding Module from Cellvibrio japonicus Xyn10C journal, August 2003

Solution Structure of the CBM10 Cellulose Binding Module from Pseudomonas Xylanase A, journal, January 2000

Pectin structure and biosynthesis journal, June 2008

The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity journal, September 2001

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules journal, October 2013

Recent insights into copper-containing lytic polysaccharide mono-oxygenases journal, October 2013

In-Frame Deletions Allow Functional Characterization of Complex Cellulose Degradation Phenotypes in Cellvibrio japonicus journal, June 2015

The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa journal, July 1988

The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel journal, August 1995

Cell-wall carbohydrates and their modification as a resource for biofuels journal, May 2008

Hemicelluloses journal, June 2010

Purification and Properties of Extracellular and Cell-bound Cellulase Components of Pseudomonas fluorescens var. cellulosa journal, January 1970

Functional and structural characterization of a potent GH 74 endo ‐xyloglucanase from the soil saprophyte Cellvibrio japonicus unravels the first step of xyloglucan degradation journal, January 2016

Understanding How the Complex Molecular Architecture of Mannan-degrading Hydrolases Contributes to Plant Cell Wall Degradation journal, December 2013

A complex gene locus enables xyloglucan utilization in the model saprophyte C ellvibrio japonicus journal, September 2014

Evidence That Galactanase A from Pseudomonas fluorescens Subspecies cellulosa Is a Retaining Family 53 Glycosyl Hydrolase in Which E161 and E270 Are the Catalytic Residues † journal, December 1997

Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31 journal, November 2012

Effect of Nature and Supply of Carbon Sources on Cellulase Formation in Pseudomonas fluorescens var. cellulosa journal, January 1970

The Membrane-Bound α-Glucuronidase from Pseudomonas cellulosa Hydrolyzes 4-O-Methyl-d-Glucuronoxylooligosaccharides but Not 4-O-Methyl-d-Glucuronoxylan journal, September 2002

Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes journal, December 1990

Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding journal, May 2001

X-ray crystallographic study of xylopentaose binding toPseudomonas fluorescens xylanase A journal, January 2000

Characterization and expression in Escherichia coli of an endoglucanase gene of Pseudomonas fluorescens subsp. cellulosa journal, July 1988

Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa journal, July 2001

Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan journal, January 1997

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli journal, November 2011

Novel bacterial ferulic acid esterase from Cellvibrio japonicus and its application in ferulic acid release and xylan hydrolysis journal, September 2010

Structural and Biochemical Analysis of Cellvibrio japonicus Xylanase 10C : HOW VARIATION IN SUBSTRATE-BINDING CLEFT INFLUENCES THE CATALYTIC PROFILE OF FAMILY GH-10 XYLANASES journal, December 2003

Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification journal, May 2011

A non-modular endo-β-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa journal, February 1995

Carbohydrate esterases of family 2 are 6- O -deacetylases journal, December 2009

Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa journal, January 1993

Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa journal, February 1995

Microbial Cellulose Utilization: Fundamentals and Biotechnology journal, September 2002

Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase journal, September 2001

The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre journal, May 1990

Structure of a Mannan-specific Family 35 Carbohydrate-Binding Module: Evidence for Significant Conformational Changes upon Ligand Binding journal, March 2005

The Structure and Function of an Arabinan-specific α-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases
journal, February 2011

A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions The GenBank accession numbers for the sequences described in this paper are AF049493 and AF168359 for xynC and xynG, respectively.
journal, August 2000

The modular architecture of Cellvibrio japonicus mannanases in glycoside hydrolase families 5 and 26 points to differences in their role in mannan degradation
journal, May 2003

The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism
journal, August 1999

Crystallization and preliminary X-ray diffraction studies of a family 26 endo-β-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa
journal, January 1998

DNA sequence analysis of endoglucanase genes fromPseudomonas fluorescens subsp.cellulosa andPseudomonas sp. NCIB 8634
journal, December 1990

The Use of Forced Protein Evolution to Investigate and Improve Stability of Family 10 Xylanases: The Production Of Ca²⁺-Independent Stable Xylanases
journal, September 2004

Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase
journal, July 1990

Crystallization and preliminary X-ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa
journal, February 1999

The resistance of cellulases and xylanases to proteolytic inactivation
journal, April 1995

The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains
journal, November 1991

A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain
journal, September 1993

Biogenesis of Multiple Cellulase Components of Pseudomonas fluorescens var. cellulosa
journal, March 1974

The Structural Basis for Catalysis and Specificity of the Pseudomonas cellulosa α-Glucuronidase, GlcA67A
journal, April 2002

Refined Crystal Structure of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens at 1.8 Å Resolution
journal, March 1996

The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions
journal, March 2009

The α-Glucuronidase, GlcA67A, of Cellvibrio japonicus Utilizes the Carboxylate and Methyl Groups of Aldobiouronic Acid as Important Substrate Recognition Determinants
journal, March 2003

Novel cellulose-binding domains, NodB homologues and conserved modular architecture in xylanases from the aerobic soil bacteria Pseudomonas fluorescens subsp. cellulosa and Cellvibrio mixtus
journal, November 1995

Mannanase A from Pseudomonas fluorescens ssp. cellulosa Is a Retaining Glycosyl Hydrolase in Which E212 and E320 Are the Putative Catalytic Residues ^†
journal, January 1996

The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics
journal, January 2009

Characterization of the cellulose-degrading bacterium NCIMB 10462
journal, September 1995

A new family of rhamnogalacturonan lyases contains an enzyme that binds to cellulose
journal, February 2001

The molecular biology of chitin digestion
journal, June 1998

Evidence for multiple carboxymethylcellulase genes in Pseudomonas fluorescens subsp. cellulosa
journal, January 1987

Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites
journal, November 1994

Starch turnover: pathways, regulation and role in growth
journal, June 2012

Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature
journal, January 2015

The carbohydrate-active enzymes database (CAZy) in 2013
journal, November 2013

Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus
journal, February 2016

Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module
journal, February 2001

The Cellvibrio japonicus Mannanase Cj Man26C Displays a Unique exo -Mode of Action That Is Conferred by Subtle Changes to the Distal Region of the Active Site
journal, September 2008

Expression of an endoglucanase gene of Pseudomonas fluorescens var. cellulosa in Zymomonas mobilis
journal, March 1988

Insights into Plant Cell Wall Degradation from the Genome Sequence of the Soil Bacterium Cellvibrio japonicus
journal, June 2008

Lytic Polysaccharide Monooxygenases in Biomass Conversion
journal, December 2015

A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes
journal, January 2014

Characterization of the gene celD and its encoded product 1,4-β-d-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa
journal, August 1992

Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo- mode of action
journal, April 1997

Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11
journal, August 2002

Pseudomonas fluorescens subsp. cellulosa : an alternative model for bacterial cellulase
journal, March 1992

Reclassification of 'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev.
journal, March 2003

Importance of Hydrophobic and Polar Residues in Ligand Binding in the Family 15 Carbohydrate-Binding Module from Cellvibrio japonicus Xyn10C
journal, August 2003

Solution Structure of the CBM10 Cellulose Binding Module from Pseudomonas Xylanase A^,
journal, January 2000

Pectin structure and biosynthesis
journal, June 2008

The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity
journal, September 2001

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules
journal, October 2013

Recent insights into copper-containing lytic polysaccharide mono-oxygenases
journal, October 2013

In-Frame Deletions Allow Functional Characterization of Complex Cellulose Degradation Phenotypes in Cellvibrio japonicus
journal, June 2015

The nucleotide sequence of a carboxymethylcellulase gene from Pseudomonas fluorescens subsp. cellulosa
journal, July 1988

The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel
journal, August 1995

Cell-wall carbohydrates and their modification as a resource for biofuels
journal, May 2008

Hemicelluloses
journal, June 2010

Purification and Properties of Extracellular and Cell-bound Cellulase Components of Pseudomonas fluorescens var. cellulosa
journal, January 1970

Functional and structural characterization of a potent GH 74 endo ‐xyloglucanase from the soil saprophyte Cellvibrio japonicus unravels the first step of xyloglucan degradation
journal, January 2016

Understanding How the Complex Molecular Architecture of Mannan-degrading Hydrolases Contributes to Plant Cell Wall Degradation
journal, December 2013

A complex gene locus enables xyloglucan utilization in the model saprophyte C ellvibrio japonicus
journal, September 2014

Evidence That Galactanase A from Pseudomonas fluorescens Subspecies cellulosa Is a Retaining Family 53 Glycosyl Hydrolase in Which E161 and E270 Are the Catalytic Residues ^†
journal, December 1997

Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31
journal, November 2012

Effect of Nature and Supply of Carbon Sources on Cellulase Formation in Pseudomonas fluorescens var. cellulosa
journal, January 1970

The Membrane-Bound α-Glucuronidase from Pseudomonas cellulosa Hydrolyzes 4-O-Methyl-d-Glucuronoxylooligosaccharides but Not 4-O-Methyl-d-Glucuronoxylan
journal, September 2002

Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes
journal, December 1990

Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding
journal, May 2001

X-ray crystallographic study of xylopentaose binding toPseudomonas fluorescens xylanase A
journal, January 2000

Characterization and expression in Escherichia coli of an endoglucanase gene of Pseudomonas fluorescens subsp. cellulosa
journal, July 1988

Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa
journal, July 2001

Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan
journal, January 1997

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli
journal, November 2011

Novel bacterial ferulic acid esterase from Cellvibrio japonicus and its application in ferulic acid release and xylan hydrolysis
journal, September 2010

Structural and Biochemical Analysis of Cellvibrio japonicus Xylanase 10C : HOW VARIATION IN SUBSTRATE-BINDING CLEFT INFLUENCES THE CATALYTIC PROFILE OF FAMILY GH-10 XYLANASES
journal, December 2003

Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification
journal, May 2011

A non-modular endo-β-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa
journal, February 1995

Carbohydrate esterases of family 2 are 6- O -deacetylases
journal, December 2009

Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa
journal, January 1993

Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa
journal, February 1995

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002

Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase
journal, September 2001

The N-terminal region of an endoglucanase from Pseudomonas fluorescens subspecies cellulosa constitutes a cellulose-binding domain that is distinct from the catalytic centre
journal, May 1990

Structure of a Mannan-specific Family 35 Carbohydrate-Binding Module: Evidence for Significant Conformational Changes upon Ligand Binding
journal, March 2005

Carbohydrate-binding modules: fine-tuning polysaccharide recognition
journal, September 2004

Cellvibrio japonicus α-L-arabinanase 43A has a novel five-blade β-propeller fold
journal, August 2002

Regulation of the Xylan-degrading Apparatus of Cellvibrio japonicus by a Novel Two-component System
journal, October 2008