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Title: Unique DNA-barcoded aerosol test particles for studying aerosol transport

Data are presented for the first use of novel DNA-barcoded aerosol test particles that have been developed to track the fate of airborne contaminants in populated environments. Until DNATrax (DNA Tagged Reagents for Aerosol eXperiments) particles were developed, there was no way to rapidly validate air transport models with realistic particles in the respirable range of 1–10 μm in diameter. The DNATrax particles, developed at Lawrence Livermore National Laboratory (LLNL) and tested with the assistance of the Pentagon Force Protection Agency, are the first safe and effective materials for aerosol transport studies that are identified by DNA molecules. The use of unique synthetic DNA barcodes overcomes the challenges of discerning the test material from pre-existing environmental or background contaminants (either naturally occurring or previously released). The DNATrax particle properties are demonstrated to have appropriate size range (approximately 1–4.5 μm in diameter) to accurately simulate bacterial spore transport. As a result, we describe details of the first field test of the DNATrax aerosol test particles in a large indoor facility.
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [3] ;  [4] ;  [4] ;  [4] ;  [1] ;  [5]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
  3. Pentagon Force Protection Agency, Arlington, VA (United States)
  4. Parsons Corp., Arlington, VA (United States)
  5. SkyeSciences, LLC, Livermore, CA (United States)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 0278-6826
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Aerosol Science and Technology
Additional Journal Information:
Journal Volume: 50; Journal Issue: 5; Journal ID: ISSN 0278-6826
American Association for Aerosol Research
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
Country of Publication:
United States