Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions

Yu, Chaowei; Reddy, Amitha P.; Simmons, Christopher W.; Simmons, Blake A.; Singer, Steven W.; VanderGheynst, Jean S.

doi:10.1186/s13068-015-0392-y

Title: Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions

Journal Article · Wed Dec 02 00:00:00 EST 2015 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-015-0392-y· OSTI ID:1618629

Yu, Chaowei; Reddy, Amitha P.; Simmons, Christopher W.; Simmons, Blake A.; Singer, Steven W.;

Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA gene sequencing of DNA extracted from enrichment samples showed that the majority of the shift in composition of the switchgrass-degrading community occurred during the initial three 2-week enrichments. Shifts in community structure upon storage occurred in all cryopreserved samples. Storage in liquid nitrogen in the absence of cryoprotectant resulted in variable preservation of dominant microorganisms in enriched samples. Cryopreservation with either DMSO or glycerol provided consistent and equivalent preservation of dominant organisms. In conclusion, a stable switchgrass-degrading microbial community was achieved after three 2-week enrichments. Dominant microorganisms were preserved equally well with DMSO and glycerol. DMSO-preserved communities required more incubation time upon revival to achieve pre-storage activity levels during high-solid thermophilic cultivation on switchgrass. Despite shifts in the community with storage, the samples were active upon revival under thermophilic and high-solid conditions. The results suggest that the presence of microorganisms may be more important than their relative abundance in retaining an active microbial community.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; CA-D-BAE-2228-RR; 12-LR-237496

OSTI ID:: 1618629

Alternate ID(s):: OSTI ID: 1257272

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 8 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 18 works

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Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions [Supplemental Data] Simmons, Christopher; Simmons, Blake; Singer, Steven https://doi.org/10.6084/m9.figshare.c.3615665 The current record is supplemented by this figure	figure	December 2015
MOESM1 of Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions Yu, Chaowei; Reddy, Amitha; Simmons, Christopher https://doi.org/10.6084/m9.figshare.c.3615665_d1 The current record is supplemented by this text	text	January 2015
MOESM1 of Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions Simmons, Christopher; Simmons, Blake; Singer, Steven https://doi.org/10.6084/m9.figshare.c.3615665_d1.v1 The current record is supplemented by this figure	figure	December 2015
Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions [Supplemental Data] Yu, Chaowei; Reddy, Amitha; Simmons, Christopher https://doi.org/10.6084/m9.figshare.c.3615665.v1 The current record is supplemented by this dataset	dataset	December 2015