skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Characterization of protein N-glycosylation by tandem mass spectrometry using complementary fragmentation techniques

Journal Article · · Frontiers in Plant Science
 [1];  [1];  [2];  [1]
  1. Univ. of Melbourne, Melbourne, VIC (Australia)
  2. Univ. of Melbourne, Melbourne, VIC (Australia); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
+ Show Author Affiliations

The analysis of post-translational modifications (PTMs) by proteomics is regarded as a technically challenging undertaking. While in recent years approaches to examine and quantify protein phosphorylation have greatly improved, the analysis of many protein modifications, such as glycosylation, are still regarded as problematic. Limitations in the standard proteomics workflow, such as use of suboptimal peptide fragmentation methods, can significantly prevent the identification of glycopeptides. The current generation of tandem mass spectrometers has made available a variety of fragmentation options, many of which are becoming standard features on these instruments. Lastly, we have used three common fragmentation techniques, namely CID, HCD, and ETD, to analyze a glycopeptide and highlight how an integrated fragmentation approach can be used to identify the modified residue and characterize the N-glycan on a peptide.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
CE110001007; FT130101165; AC02-05CH11231
OSTI ID:
1257263
Journal Information:
Frontiers in Plant Science, Vol. 6; ISSN 1664-462X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 22 works
Citation information provided by
Web of Science

References (49)

Asparagus Spears as a Model to Study Heteroxylan Biosynthesis during Secondary Wall Development journal April 2015
On-Line LC−MS Approach Combining Collision-Induced Dissociation (CID), Electron-Transfer Dissociation (ETD), and CID of an Isolated Charge-Reduced Species for the Trace-Level Characterization of Proteins with Post-Translational Modifications journal November 2007
A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases journal May 2007
N-glycoproteomics in plants: Perspectives and challenges journal August 2011
The N-glycans of jack bean alpha-mannosidase. Structure, topology and function journal August 1999
The protein N-glycosylation in plants journal September 1998
MASCP gator: an overview of the Arabidopsis proteomic aggregation portal journal January 2013
Effects of glycosylation on protein folding, stability and solubility. Studies of chemically modified or engineered plant and fungal peroxidases book January 1995
Electron transfer dissociation ofN-glycopeptides: loss of the entireN-glycosylated asparagine side chain
  • Catalina, M. Isabel; Koeleman, Carolien A. M.; Deelder, André M.
  • Rapid Communications in Mass Spectrometry, Vol. 21, Issue 6 https://doi.org/10.1002/rcm.2929
journal January 2007
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry journal June 2004
Higher-energy C-trap dissociation for peptide modification analysis journal August 2007
Concepts and Principles of O-Linked Glycosylation journal January 1998
Effect of genetic background on glycosylation heterogeneity in human antithrombin produced in the mammary gland of transgenic goats journal April 2005
Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry journal February 2007
Characterization of glycopeptides by combining collision-induced dissociation and electron-transfer dissociation mass spectrometry data journal January 2009
Identification of loss-of-function mutations of SLC35D1 in patients with Schneckenbecken dysplasia, but not with other severe spondylodysplastic dysplasias group diseases journal June 2009
Application of electron transfer dissociation (ETD) for the analysis of posttranslational modifications journal November 2008
Combining various strategies to increase the coverage of the plant cell wall glycoproteome journal July 2011
The Green Proteome: Challenges in Plant Proteomics journal January 2011
Software for automated interpretation of mass spectrometry data from glycans and glycopeptides journal January 2013
Isolation of N - Linked Glycopeptides from Plasma journal August 2007
Increasing the Productivity of Glycopeptides Analysis by Using Higher-Energy Collision Dissociation-Accurate Mass-Product-Dependent Electron Transfer Dissociation journal January 2012
Glycopeptide Analysis, Recent Developments and Applications journal February 2013
Ion activation methods for tandem mass spectrometry journal January 2004
Simultaneous Glycan-Peptide Characterization Using Hydrophilic Interaction Chromatography and Parallel Fragmentation by CID, Higher Energy Collisional Dissociation, and Electron Transfer Dissociation MS Applied to the N -Linked Glycoproteome of Campylobacter jejuni journal April 2010
Automated glycopeptide analysis--review of current state and future directions journal July 2012
Analysis of protein glycosylation by mass spectrometry journal June 2007
The Secreted Plant N-Glycoproteome and Associated Secretory Pathways journal January 2012
Mapping N-Glycosylation Sites across Seven Evolutionarily Distant Species Reveals a Divergent Substrate Proteome Despite a Common Core Machinery journal May 2012
Biological significance of complex N-glycans in plants and their impact on plant physiology journal July 2014
Towards characterization of the glycoproteome of tomato (Solanum lycopersicum) fruit using Concanavalin A lectin affinity chromatography and LC-MALDI-MS/MS analysis journal March 2011
Analytical technologies for identification and characterization of the plant N-glycoproteome journal January 2012
Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics journal December 2011
Plant-specific glycosylation patterns in the context of therapeutic protein production: PMP-specific glycosylation patterns journal March 2010
Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites book January 2006
Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry journal May 2003
Mutants in DEFECTIVE GLYCOSYLATION, an Arabidopsis homolog of an oligosaccharyltransferase complex subunit, show protein underglycosylation and defects in cell differentiation and growth: DGL1 is required for protein N-glycosylation journal April 2005
A Systematic Investigation into the Nature of Tryptic HCD Spectra journal October 2012
The Effects of the Site-Directed Removal of N-Glycosylation from Cationic Peanut Peroxidase on Its Function journal February 2001
Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process journal April 1998
Glycosylation: Heterogeneity and the 3D Structure of Proteins journal January 1997
N-glycan occupancy of Arabidopsis N-glycoproteins journal November 2013
Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F cannot release glycans with fucose attached alpha1 3 to the asparagine-linked N-acetylglucosamine residue journal August 1991
A simple cellulose column procedure for selective enrichment of glycopeptides and characterization by nano LC coupled with electron-transfer and high-energy collisional-dissociation tandem mass spectrometry journal April 2010
Comparison of CID versus ETD based MS/MS fragmentation for the analysis of protein ubiquitination journal September 2009
Cell wall O-glycoproteins and N-glycoproteins: aspects of biosynthesis and function journal October 2014
Proteomic analysis of glycosylation: structural determination of N - and O -linked glycans by mass spectrometry journal January 2005
Characterization of the Arabinogalactan Protein 31 (AGP31) of Arabidopsis thaliana: NEW ADVANCES ON THE HYP-O-GLYCOSYLATION OF THE PRO-RICH DOMAIN journal January 2012
Characterization of the glycosylation sites in yeast external invertase. I. N-linked oligosaccharide content of the individual sequons. journal May 1988

Cited By (5)

The Role of Electron Transfer Dissociation in Modern Proteomics journal December 2017
Plants as Factories for Human Pharmaceuticals: Applications and Challenges journal December 2015
Structure and effective charge characterization of proteins by a mobility capillary electrophoresis based method journal January 2019
Characterization of glycopeptides using a stepped higher-energy C-trap dissociation approach on a hybrid quadrupole orbitrap journal July 2018
The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes journal March 2018

Similar Records

Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation
Journal Article · Wed Jan 15 00:00:00 EST 2014 · Analytical Biochemistry, 452:96-102 · OSTI ID:1257263
+8 more
Improving CID, HCD, and ETD FT MS/MS degradome-peptidome identifications using high accuracy mass information
Journal Article · Mon Nov 07 00:00:00 EST 2011 · Journal of Proteome Research · OSTI ID:1257263
+1 more
N-Glycopeptide Profiling in Arabidopsis Inflorescence
Journal Article · Mon Apr 11 00:00:00 EDT 2016 · Molecular and Cellular Proteomics · OSTI ID:1257263
+2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
glycosylation
ragmentation
electron-transfer dissociation
post-translational modification
tandem mass spectrometry