Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

Cheng, Qingsu; Aravind, Ashwin; Buckley, Matthew; Gifford, Andrew; Parvin, Bahram

doi:10.1038/srep13685

Title: Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments

Journal Article · Tue Sep 08 00:00:00 EDT 2015 · Scientific Reports

DOI:https://doi.org/10.1038/srep13685· OSTI ID:1255532

Cheng, Qingsu ^[1]; Aravind, Ashwin ^[2]; Buckley, Matthew ^[2]; Gifford, Andrew ^[3]; Parvin, Bahram ^[1]

Univ. of Nevada, Reno, NV (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)

To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. In conclusion, we also demonstrate that cysteine-functionalized C60- pyrrolidine tris acid can differentiate live and dead microorganisms.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1255532

Journal Information:: Scientific Reports, Vol. 5; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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