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Title: Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids

Human biofluids, especially blood plasma or serum, hold great potential as the sources of potential biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge to detect promising low-abundance protein biomarkers. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high and moderate-abundance proteins, thus enabling more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomics applications of different human biofluids. The limitations and future perspective of immunoaffinity separation methods are also discussed.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
1255402
Report Number(s):
PNNL-SA-112289
Journal ID: ISSN 1570-0232; 48135; 46197; 40991; 48666; 453040220
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Chromatography. B; Journal Volume: 1021
Publisher:
Elsevier
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory