Vacuum Ultraviolet Photodissociation and Fourier Transform–Ion Cyclotron Resonance (FT-ICR) Mass Spectrometry: Revisited

Journal Article · Tue Feb 16 00:00:00 EST 2016 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.6b00148· OSTI ID:1255397

Shaw, Jared B.; Robinson, Errol W.; Pasa-Tolic, Ljiljana

We revisited the implementation of UVPD within the ICR cell of a FT-ICR mass spectrometer. UVPD performance characteristics were examined in the context of recent developments in the understanding of UVPD and in-cell tandem mass spectrometry. Efficient UVPD and photo-ECD of a model peptide and small protein within the ICR cell of a FT-ICR mass spectrometer are accomplished through appropriate modulation of laser pulse timing relative to ion magnetron motion and the potential applied to an ion optical element that photons impinge on. It is shown that UVPD yields efficient and extensive fragmentation resulting in excellent sequence coverage for model peptide and protein cations.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1255397
• Report Number(s): PNNL-SA-114531; 48691; KP1704020
• Journal Information: Analytical Chemistry, Vol. 88, Issue 6; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: United States
• Language: English

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Environmental Molecular Sciences Laboratory