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Title: Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform

Characterizing protein-ligand binding dynamics is crucial for understanding protein function and developing new therapeutic agents. We have developed a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and on-chip electrospray ionization to perform label-free, solution-based monitoring of protein-ligand binding dynamics. This platform offers many advantages including automated processing, rapid mixing, and low sample consumption.
Authors:
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Publication Date:
OSTI Identifier:
1254561
Report Number(s):
PNNL-SA-115886
Journal ID: ISSN 1473-0197; 49167
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Lab on a chip (Print); Journal Volume: 16; Journal Issue: 9
Publisher:
Royal Society of Chemistry
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
Mass spectrometry; protein-ligand binding; microfluidic; Environmental Molecular Sciences Laboratory