Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants

Davern, Sandra M.; McKnight, Timothy E.; Standaert, Robert F.; Morrell-Falvey, Jennifer L.; Shpak, Elena D.; Kalluri, Udaya C.; Jelenska, Joanna; Greenberg, Jean T.; Mirzadeh, Saed; Devarenne, ed., Timothy P.

doi:10.1371/journal.pone.0153621

Title: Carbon Nanofiber Arrays: A Novel Tool for Microdelivery of Biomolecules to Plants

Journal Article · Wed Apr 27 00:00:00 EDT 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0153621· OSTI ID:1346600

Davern, Sandra M.; McKnight, Timothy E.;

; Morrell-Falvey, Jennifer L.; Shpak, Elena D.; Kalluri, Udaya C.; Jelenska, Joanna; Greenberg, Jean T.; Mirzadeh, Saed; Devarenne, ed., Timothy P.

Effective methods for delivering bioprobes into the cells of intact plants are essential for investigating diverse biological processes. Increasing research on trees, such as Populus spp., for bioenergy applications is driving the need for techniques that work well with tree species. This report introduces vertically aligned carbon nanofiber (VACNF) arrays as a new tool for microdelivery of labeled molecules to Populus leaf tissue and whole plants. We demonstrated that VACNFs penetrate the leaf surface to deliver sub-microliter quantities of solution containing fluorescent or radiolabeled molecules into Populus leaf cells. Importantly, VACNFs proved to be gentler than abrasion with carborundum, a common way to introduce material into leaves. Unlike carborundum, VACNFs did not disrupt cell or tissue integrity, nor did they induce production of hydrogen peroxide, a typical wound response. We show that femtomole to picomole quantities of labeled molecules (fluorescent dyes, small proteins and dextran), ranging from 0.5–500 kDa, can be introduced by VACNFs, and we demonstrate the use of the approach to track delivered probes from their site of introduction on the leaf to distal plant regions. VACNF arrays thus offer an attractive microdelivery method for the introduction of biomolecules and other probes into trees and potentially other types of plants.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: ERKP-417; AC05-00OR22725

OSTI ID:: 1346600

Alternate ID(s):: OSTI ID: 1254081

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 11 Journal Issue: 4; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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