Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch

Svidritskiy, Egor; Korostelev, Andrei A.

doi:10.1016/j.str.2015.08.011

Title: Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch

Journal Article · Thu Sep 24 00:00:00 EDT 2015 · Structure

DOI:https://doi.org/10.1016/j.str.2015.08.011· OSTI ID:1252757

Svidritskiy, Egor ^[1]; Korostelev, Andrei A. ^[1]

Univ. of Massachusetts Medical School, Worcester, MA (United States)

Translation initiation in the P site occasionally occurs at atypical (non-AUG) start codons, including those forming a mismatch in the third (wobble) position. During elongation, however, a pyrimidine-pyrimidine wobble mismatch may trigger a translation quality-control mechanism, whereby the P-site mismatch is thought to perturb the downstream A-site codon or the decoding center, thereby reducing translation fidelity and inducing termination of aberrant translation. We report a crystal structure of the 70S initiation complex containing an AUC codon in the ribosomal P site. Remarkably, the ribosome stabilizes the mismatched codon-anticodon helix, arranging a normally disruptive cytosine-cytosine pair into a Watson-Crick-like conformation. Here, the translation-competent conformations of the tRNA, mRNA, and decoding center suggest that a P-site wobble-position mismatch in the 70S initiation complex does not pre-arrange the mRNA or decoding center to favor subsequent miscoding events.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH)

Grant/Contract Number:: GM106105; GM107465

OSTI ID:: 1252757

Journal Information:: Structure, Vol. 23, Issue 11; ISSN 0969-2126

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 10 works

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