Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids

Wu, Chaochao; Duan, Jicheng; Liu, Tao; Smith, Richard D.; Qian, Wei -Jun

doi:10.1016/j.jchromb.2016.01.015

Title: Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids

Journal Article · Tue Jan 12 00:00:00 EST 2016 · Journal of Chromatography. B

DOI:https://doi.org/10.1016/j.jchromb.2016.01.015· OSTI ID:1251560

Wu, Chaochao ^[1];

^[1]; Liu, Tao ^[1]; Smith, Richard D. ^[1];

^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high- and moderate-abundance proteins, thus enabling much more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomic applications in human biofluids. The limitations and future perspectives of immunoaffinity separation methods are also discussed.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1251560

Report Number(s):: PNNL-SA-112289; PII: S1570023216300150

Journal Information:: Journal of Chromatography. B, Vol. 1021, Issue C; ISSN 1570-0232

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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