Synthetic biology for microbial production of lipid-based biofuels

d’Espaux, Leo; Mendez-Perez, Daniel; Li, Rachel; Keasling, Jay D.

doi:10.1016/j.cbpa.2015.09.009

Title: Synthetic biology for microbial production of lipid-based biofuels

Journal Article · Fri Oct 23 00:00:00 EDT 2015 · Current Opinion in Chemical Biology

DOI:https://doi.org/10.1016/j.cbpa.2015.09.009· OSTI ID:1379004

d’Espaux, Leo ^[1]; Mendez-Perez, Daniel ^[1]; Li, Rachel ^[2]; Keasling, Jay D. ^[3]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering, QB3 Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering, QB3 Inst.

The risks of maintaining current CO₂ emission trends have led to interest in producing biofuels using engineered microbes. Microbial biofuels reduce emissions because CO₂ produced by fuel combustion is offset by CO2 captured by growing biomass, which is later used as feedstock for biofuel fermentation. Hydrocarbons found in petroleum fuels share striking similarity with biological lipids. Here in this paper we review synthetic metabolic pathways based on fatty acid and isoprenoid metabolism to produce alkanes and other molecules suitable as biofuels. Lastly, we further discuss engineering strategies to optimize engineered biosynthetic routes, as well as the potential of synthetic biology for sustainable manufacturing.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; MCB-1341894

OSTI ID:: 1379004

Alternate ID(s):: OSTI ID: 1251463

Journal Information:: Current Opinion in Chemical Biology, Vol. 29, Issue C; ISSN 1367-5931

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 50 works

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