Structure and Function of Fusicoccadiene Synthase, a Hexameric Bifunctional Diterpene Synthase

Chen, Mengbin; Chou, Wayne K. W.; Toyomasu, Tomonobu; Cane, David E.; Christianson, David W.

doi:10.1021/acschembio.5b00960

Title: Structure and Function of Fusicoccadiene Synthase, a Hexameric Bifunctional Diterpene Synthase

Journal Article · Wed Jan 06 00:00:00 EST 2016 · ACS Chemical Biology

DOI:https://doi.org/10.1021/acschembio.5b00960· OSTI ID:1249236

Chen, Mengbin ^[1]; Chou, Wayne K. W. ^[2]; Toyomasu, Tomonobu ^[3]; Cane, David E. ^[2]; Christianson, David W. ^[4]

Univ. of Pennsylvania, Philadelphia, PA (United States)
Brown Univ., Providence, RI (United States)
Yamagata Univ. (Japan)
Univ. of Pennsylvania, Philadelphia, PA (United States); Harvard Univ., Cambridge, MA (United States)

Fusicoccin A is a diterpene glucoside phytotoxin generated by the fungal pathogen Phomopsis amygdali that causes the plant disease constriction canker, first discovered in New Jersey peach orchards in the 1930s. Fusicoccin A is also an emerging new lead in cancer chemotherapy. The hydrocarbon precursor of fusicoccin A is the tricyclic diterpene fusicoccadiene, which is generated by a bifunctional terpenoid synthase. Here, we report X-ray crystal structures of the individual catalytic domains of fusicoccadiene synthase: the C-terminal domain is a chain elongation enzyme that generates geranylgeranyl diphosphate, and the N-terminal domain catalyzes the cyclization of geranylgeranyl diphosphate to form fusicoccadiene. Here, crystal structures of each domain complexed with bisphosphonate substrate analogues suggest that three metal ions and three positively charged amino acid side chains trigger substrate ionization in each active site. While in vitro incubations reveal that the cyclase domain can utilize farnesyl diphosphate and geranyl diphosphate as surrogate substrates, these shorter isoprenoid diphosphates are mainly converted into acyclic alcohol or hydrocarbon products. Gel filtration chromatography and analytical ultracentrifugation experiments indicate that full-length fusicoccadiene synthase adopts hexameric quaternary structure, and small-angle X-ray scattering data yield a well-defined molecular envelope illustrating a plausible model for hexamer assembly.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357; P41 GM103403; S10 RR029205

OSTI ID:: 1249236

Journal Information:: ACS Chemical Biology, Vol. 11, Issue 4; ISSN 1554-8929

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 39 works

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Protonation-Initiated Cyclization by a Class II Terpene Cyclase Assisted by Tunneling Eriksson, Adam; Kürten, Charlotte; Syrén, Per-Olof ChemBioChem, Vol. 18, Issue 23 https://doi.org/10.1002/cbic.201700443	journal	November 2017
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Unearthing a sesterterpene biosynthetic repertoire in the Brassicaceae through genome mining reveals convergent evolution Huang, Ancheng C.; Kautsar, Satria A.; Hong, Young J. Proceedings of the National Academy of Sciences, Vol. 114, Issue 29 https://doi.org/10.1073/pnas.1705567114	journal	July 2017
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