Elastic properties of protein functionalized nanoporous polymer films

Wang, Haoyu; Akcora, Pinar

doi:10.1021/acs.langmuir.5b04334

Title: Elastic properties of protein functionalized nanoporous polymer films

Journal Article · Wed Dec 16 00:00:00 EST 2015 · Langmuir

DOI:https://doi.org/10.1021/acs.langmuir.5b04334· OSTI ID:1246790

Wang, Haoyu ^[1]; Akcora, Pinar ^[1]

Stevens Institute of Technology, Hoboken, NJ (United States)

Retaining the conformational structure and bioactivity of immobilized proteins is important for biosensor designs and drug delivery systems. Confined environments often lead to changes in conformation and functions of proteins. In this study, lysozyme is chemically tethered into nanopores of polystyrene thin films, and submicron pores in poly(methyl methacrylate) films are functionalized with streptavidin. Nanoindentation experiments show that stiffness of streptavidin increases with decreasing submicron pore sizes. Lysozymes in polystyrene nanopores are found to behave stiffer than the submicron pore sizes and still retain their specific bioactivity relative to the proteins on flat surfaces. Lastly, our results show that protein functionalized ordered nanoporous polystyrene/poly(methyl methacrylate) films present heterogeneous elasticity and can be used to study interactions between free proteins and designed surfaces.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC00112704

OSTI ID:: 1246790

Report Number(s):: BNL-111949-2016-JA; R&D Project: 16062/16062; KC0403020

Journal Information:: Langmuir, Vol. 32, Issue 1; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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36 MATERIALS SCIENCE
nanoporous polystyrene
poly(methyl methacrylate)
thin film
lysozyme
stiffness
activity
heterogeneous surface

Title: Elastic properties of protein functionalized nanoporous polymer films

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