Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies
Abstract
Olfactomedin (OLF) domains are found within extracellular, multidomain proteins in numerous tissues of multicellular organisms. Even though these proteins have been implicated in human disorders ranging from cancers to attention deficit disorder to glaucoma, little is known about their structure(s) and function(s). Here we biophysically, biochemically, and structurally characterize OLF domains from H. sapiens olfactomedin-1 (npoh-OLF, also called noelin, pancortin, OLFM1, and hOlfA), and M. musculus gliomedin (glio-OLF, also called collomin, collmin, and CRG-L2), and compare them with available structures of myocilin (myoc-OLF) recently reported by us and R. norvegicus glio-OLF and M. musculus latrophilin-3 (lat3-OLF) by others. Although the five-bladed β-propeller architecture remains unchanged, numerous physicochemical characteristics differ among these OLF domains. First, npoh-OLF and glio-OLF exhibit prominent, yet distinct, positive surface charges and copurify with polynucleotides. Second, whereas npoh-OLF and myoc-OLF exhibit thermal stabilities typical of human proteins near 55°C, and most myoc-OLF variants are destabilized and highly prone to aggregation, glio-OLF is nearly 20°C more stable and significantly more resistant to chemical denaturation. Phylogenetically, glio-OLF is most similar to primitive OLFs, and structurally, glio-OLF is missing distinguishing features seen in OLFs such as the disulfide bond formed by N- and C- terminal cysteines, the sequestered Ca2+ ionmore »
- Authors:
-
- Georgia Inst. of Technology, Atlanta, GA (United States)
- Univ. of Iowa, Iowa City, IA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health
- OSTI Identifier:
- 1245865
- Grant/Contract Number:
- R01EY021205; W-31-109-Eng-38
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 6; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Protein domains; Crystal structure; Heparin; Binding analysis; Molecular structure; Glaucoma; Thermal stability; Disulfide bonds
Citation Formats
Hill, Shannon E., Donegan, Rebecca K., Nguyen, Elaine, Desai, Tanay M., Lieberman, Raquel L., and Anderson, Michael G. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. United States: N. p., 2015.
Web. doi:10.1371/journal.pone.0130888.
Hill, Shannon E., Donegan, Rebecca K., Nguyen, Elaine, Desai, Tanay M., Lieberman, Raquel L., & Anderson, Michael G. Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies. United States. https://doi.org/10.1371/journal.pone.0130888
Hill, Shannon E., Donegan, Rebecca K., Nguyen, Elaine, Desai, Tanay M., Lieberman, Raquel L., and Anderson, Michael G. 2015.
"Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies". United States. https://doi.org/10.1371/journal.pone.0130888. https://www.osti.gov/servlets/purl/1245865.
@article{osti_1245865,
title = {Molecular Details of Olfactomedin Domains Provide Pathway to Structure-Function Studies},
author = {Hill, Shannon E. and Donegan, Rebecca K. and Nguyen, Elaine and Desai, Tanay M. and Lieberman, Raquel L. and Anderson, Michael G.},
abstractNote = {Olfactomedin (OLF) domains are found within extracellular, multidomain proteins in numerous tissues of multicellular organisms. Even though these proteins have been implicated in human disorders ranging from cancers to attention deficit disorder to glaucoma, little is known about their structure(s) and function(s). Here we biophysically, biochemically, and structurally characterize OLF domains from H. sapiens olfactomedin-1 (npoh-OLF, also called noelin, pancortin, OLFM1, and hOlfA), and M. musculus gliomedin (glio-OLF, also called collomin, collmin, and CRG-L2), and compare them with available structures of myocilin (myoc-OLF) recently reported by us and R. norvegicus glio-OLF and M. musculus latrophilin-3 (lat3-OLF) by others. Although the five-bladed β-propeller architecture remains unchanged, numerous physicochemical characteristics differ among these OLF domains. First, npoh-OLF and glio-OLF exhibit prominent, yet distinct, positive surface charges and copurify with polynucleotides. Second, whereas npoh-OLF and myoc-OLF exhibit thermal stabilities typical of human proteins near 55°C, and most myoc-OLF variants are destabilized and highly prone to aggregation, glio-OLF is nearly 20°C more stable and significantly more resistant to chemical denaturation. Phylogenetically, glio-OLF is most similar to primitive OLFs, and structurally, glio-OLF is missing distinguishing features seen in OLFs such as the disulfide bond formed by N- and C- terminal cysteines, the sequestered Ca2+ ion within the propeller central hydrophilic cavity, and a key loop-stabilizing cation-π interaction on the top face of npoh-OLF and myoc-OLF. While deciphering the explicit biological functions, ligands, and binding partners for OLF domains will likely continue to be a challenging long-term experimental pursuit, we used structural insights gained here to generate a new antibody selective for myoc-OLF over npoh-OLF and glio-OLF as a first step in overcoming the impasse in detailed functional characterization of these biomedically important protein domains.},
doi = {10.1371/journal.pone.0130888},
url = {https://www.osti.gov/biblio/1245865},
journal = {PLoS ONE},
issn = {1932-6203},
number = 6,
volume = 10,
place = {United States},
year = {Mon Jun 29 00:00:00 EDT 2015},
month = {Mon Jun 29 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Identification of a Gene That Causes Primary Open Angle Glaucoma
journal, January 1997
- Stone, E. M.
- Science, Vol. 275, Issue 5300
Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001
- Baker, N. A.; Sept, D.; Joseph, S.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041
Genetic dissection of myocilin glaucoma
journal, January 2004
- Gong, G.
- Human Molecular Genetics, Vol. 13, Issue 90001
Structural Basis of Latrophilin-FLRT Interaction
journal, April 2015
- Jackson, Verity A.; del Toro, Daniel; Carrasquero, Maria
- Structure, Vol. 23, Issue 4
DISPLAR: an accurate method for predicting DNA-binding sites on protein surfaces
journal, February 2007
- Tjong, Harianto; Zhou, Huan-Xiang
- Nucleic Acids Research, Vol. 35, Issue 5
Noelin-1 is a secreted glycoprotein involved in generation of the neural crest
journal, March 2000
- Barembaum, Meyer; Moreno, Tanya A.; LaBonne, Carole
- Nature Cell Biology, Vol. 2, Issue 4
Exploiting the interaction between Grp94 and aggregated myocilin to treat glaucoma
journal, July 2014
- Stothert, Andrew R.; Suntharalingam, Amirthaa; Huard, Dustin J. E.
- Human Molecular Genetics, Vol. 23, Issue 24
Olfactomedin Domain-Containing Proteins: Possible Mechanisms of Action and Functions in Normal Development and Pathology
journal, June 2009
- Tomarev, Stanislav I.; Nakaya, Naoki
- Molecular Neurobiology, Vol. 40, Issue 2
A Cellular Assay Distinguishes Normal and Mutant TIGR/Myocilin Protein
journal, November 1999
- Zhou, Z.; Vollrath, D.
- Human Molecular Genetics, Vol. 8, Issue 12
The Stability of Myocilin Olfactomedin Domain Variants Provides New Insight into Glaucoma as a Protein Misfolding Disorder
journal, July 2011
- Burns, J. Nicole; Turnage, Katherine C.; Walker, Chandler A.
- Biochemistry, Vol. 50, Issue 26
Olfactomedin 4 Inhibits Cathepsin C-Mediated Protease Activities, Thereby Modulating Neutrophil Killing of Staphylococcus aureus and Escherichia coli in Mice
journal, July 2012
- Liu, Wenli; Yan, Ming; Liu, Yueqin
- The Journal of Immunology, Vol. 189, Issue 5
Characterization of extracellular circulating microRNA
journal, May 2011
- Turchinovich, Andrey; Weiz, Ludmila; Langheinz, Anne
- Nucleic Acids Research, Vol. 39, Issue 16
Glaucoma-associated myocilin: A better understanding but much more to learn
journal, April 2009
- Resch, Zachary T.; Fautsch, Michael P.
- Experimental Eye Research, Vol. 88, Issue 4
Zebrafish Olfactomedin 1 Regulates Retinal Axon Elongation In Vivo and Is a Modulator of Wnt Signaling Pathway
journal, July 2008
- Nakaya, N.; Lee, H. -S.; Takada, Y.
- Journal of Neuroscience, Vol. 28, Issue 31
Pancortin-2 Interacts with WAVE1 and Bcl-xL in a Mitochondria-Associated Protein Complex That Mediates Ischemic Neuronal Death
journal, February 2007
- Cheng, A.; Arumugam, T. V.; Liu, D.
- Journal of Neuroscience, Vol. 27, Issue 7
The Glaucoma-Associated Olfactomedin Domain of Myocilin Forms Polymorphic Fibrils That Are Constrained by Partial Unfolding and Peptide Sequence
journal, February 2014
- Hill, Shannon E.; Donegan, Rebecca K.; Lieberman, Raquel L.
- Journal of Molecular Biology, Vol. 426, Issue 4
Pancortins interact with amyloid precursor protein and modulate cortical cell migration
journal, September 2012
- Rice, H. C.; Townsend, M.; Bai, J.
- Development, Vol. 139, Issue 21
A common variant of the latrophilin 3 gene, LPHN3, confers susceptibility to ADHD and predicts effectiveness of stimulant medication
journal, February 2010
- Arcos-Burgos, M.; Jain, M.; Acosta, M. T.
- Molecular Psychiatry, Vol. 15, Issue 11
Increasing the hydrophobic interaction between terminal W-motifs enhances the stability of Salmonella typhimurium sialidase. A general strategy for the stabilization of β-propeller protein fold
journal, November 2001
- Witarto, Arief Budi; Sode, Koji
- Protein Engineering, Design and Selection, Vol. 14, Issue 11
The many blades of the β-propeller proteins: conserved but versatile
journal, October 2011
- Chen, Cammy K. -M.; Chan, Nei-Li; Wang, Andrew H. -J.
- Trends in Biochemical Sciences, Vol. 36, Issue 10
ESPript/ENDscript: extracting and rendering sequence and 3D information from atomic structures of proteins
journal, July 2003
- Gouet, P.
- Nucleic Acids Research, Vol. 31, Issue 13
Hormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cells
journal, November 2010
- Kodithuwakku, S. P.; Ng, P. -Y.; Liu, Y.
- Human Reproduction, Vol. 26, Issue 1
Increased expression of olfactomedin-1 and myocilin in podocytes during puromycin aminonucleoside nephrosis
journal, June 2010
- Bohr, D. C.; Koch, M.; Kritzenberger, M.
- Nephrology Dialysis Transplantation, Vol. 26, Issue 1
The Olfactomedin Domain from Gliomedin Is a β-Propeller with Unique Structural Properties
journal, December 2014
- Han, Huijong; Kursula, Petri
- Journal of Biological Chemistry, Vol. 290, Issue 6
Glucose-regulated Protein 94 Triage of Mutant Myocilin through Endoplasmic Reticulum-associated Degradation Subverts a More Efficient Autophagic Clearance Mechanism
journal, October 2012
- Suntharalingam, Amirthaa; Abisambra, Jose F.; O'Leary, John C.
- Journal of Biological Chemistry, Vol. 287, Issue 48
Ligands for Glaucoma-Associated Myocilin Discovered by a Generic Binding Assay
journal, November 2013
- Orwig, Susan D.; Chi, Pamela V.; Du, Yuhong
- ACS Chemical Biology, Vol. 9, Issue 2
Long-Term Maintenance of Na+ Channels at Nodes of Ranvier Depends on Glial Contact Mediated by Gliomedin and NrCAM
journal, April 2014
- Amor, V.; Feinberg, K.; Eshed-Eisenbach, Y.
- Journal of Neuroscience, Vol. 34, Issue 15
Biology of purinergic signalling: Its ancient evolutionary roots, its omnipresence and its multiple functional significance: Prospects & Overviews
journal, April 2014
- Verkhratsky, Alexei; Burnstock, Geoffrey
- BioEssays, Vol. 36, Issue 7
MicroRNAs Shape the Neuronal Landscape
journal, August 2012
- McNeill, Elizabeth; Van Vactor, David
- Neuron, Vol. 75, Issue 3
PIC: Protein Interactions Calculator
journal, May 2007
- Tina, K. G.; Bhadra, R.; Srinivasan, N.
- Nucleic Acids Research, Vol. 35, Issue Web Server
The Glaucoma-associated Olfactomedin Domain of Myocilin Is a Novel Calcium Binding Protein
journal, November 2012
- Donegan, Rebecca K.; Hill, Shannon E.; Turnage, Katherine C.
- Journal of Biological Chemistry, Vol. 287, Issue 52
The beta bulge: a common small unit of nonrepetitive protein structure.
journal, June 1978
- Richardson, J. S.; Getzoff, E. D.; Richardson, D. C.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 6
Docking Server for the Identification of Heparin Binding Sites on Proteins
journal, July 2014
- Mottarella, Scott E.; Beglov, Dmitri; Beglova, Natalia
- Journal of Chemical Information and Modeling, Vol. 54, Issue 7
Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
journal, December 1983
- Kabsch, Wolfgang; Sander, Christian
- Biopolymers, Vol. 22, Issue 12
PROMALS3D: a tool for multiple protein sequence and structure alignments
journal, February 2008
- Pei, Jimin; Kim, Bong-Hyun; Grishin, Nick V.
- Nucleic Acids Research, Vol. 36, Issue 7
Elucidation of subfamily segregation and intramolecular coevolution of the olfactomedin-like proteins by comprehensive phylogenetic analysis and gene expression pattern assessment
journal, September 2005
- Zeng, Ling-Chun; Han, Ze-Guang; Ma, Wei-Jun
- FEBS Letters, Vol. 579, Issue 25
SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
journal, January 1997
- Guex, Nicolas; Peitsch, Manuel C.
- Electrophoresis, Vol. 18, Issue 15, p. 2714-2723
Secreted gliomedin is a perinodal matrix component of peripheral nerves
journal, May 2007
- Eshed, Yael; Feinberg, Konstantin; Carey, David J.
- The Journal of Cell Biology, Vol. 177, Issue 3
Structural basis for misfolding in myocilin-associated glaucoma
journal, December 2014
- Donegan, Rebecca K.; Hill, Shannon E.; Freeman, Dana M.
- Human Molecular Genetics, Vol. 24, Issue 8
Myocilin allele-specific glaucoma phenotype database
journal, February 2008
- Hewitt, Alex W.; Mackey, David A.; Craig, Jamie E.
- Human Mutation, Vol. 29, Issue 2
Biophysical Characterization of the Olfactomedin Domain of Myocilin, an Extracellular Matrix Protein Implicated in Inherited Forms of Glaucoma
journal, January 2011
- Orwig, Susan D.; Lieberman, Raquel L.
- PLoS ONE, Vol. 6, Issue 1
A genome-wide association meta-analysis identifies new childhood obesity loci
journal, April 2012
- Early Growth Genetics (EGG) Consortium,
- Nature Genetics, Vol. 44, Issue 5, p. 526-531
Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD
journal, May 2012
- Gersemann, Michael; Becker, Svetlana; Nuding, Sabine
- Journal of Crohn's and Colitis, Vol. 6, Issue 4
Amyloid Fibril Formation by the Glaucoma-Associated Olfactomedin Domain of Myocilin
journal, August 2012
- Orwig, Susan D.; Perry, Christopher W.; Kim, Laura Y.
- Journal of Molecular Biology, Vol. 421, Issue 2-3
Rescue of Glaucoma-Causing Mutant Myocilin Thermal Stability by Chemical Chaperones
journal, March 2010
- Burns, J. Nicole; Orwig, Susan D.; Harris, Julia L.
- ACS Chemical Biology, Vol. 5, Issue 5
Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice
journal, November 2014
- Sultana, Afia; Nakaya, Naoki; Dong, Lijin
- Experimental Neurology, Vol. 261
Olfactomedin-1 activity identifies a cell invasion checkpoint during epithelial-mesenchymal transition in the chick embryonic heart
journal, December 2012
- Lencinas, A.; Chhun, D. C.; Dan, K. P.
- Disease Models & Mechanisms, Vol. 6, Issue 3
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
journal, December 1983
- Kabsch, Wolfgang; Sander, Christian
- Biopolymers, Vol. 22, Issue 12
SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
journal, January 1997
- Guex, Nicolas; Peitsch, Manuel C.
- Electrophoresis, Vol. 18, Issue 15, p. 2714-2723
Myocilin allele-specific glaucoma phenotype database
journal, February 2008
- Hewitt, Alex W.; Mackey, David A.; Craig, Jamie E.
- Human Mutation, Vol. 29, Issue 2
Olfactomedin Domain-Containing Proteins: Possible Mechanisms of Action and Functions in Normal Development and Pathology
journal, June 2009
- Tomarev, Stanislav I.; Nakaya, Naoki
- Molecular Neurobiology, Vol. 40, Issue 2
Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD
journal, May 2012
- Gersemann, Michael; Becker, Svetlana; Nuding, Sabine
- Journal of Crohn's and Colitis, Vol. 6, Issue 4
Glaucoma-associated myocilin: A better understanding but much more to learn
journal, April 2009
- Resch, Zachary T.; Fautsch, Michael P.
- Experimental Eye Research, Vol. 88, Issue 4
Deletion of olfactomedin 2 induces changes in the AMPA receptor complex and impairs visual, olfactory, and motor functions in mice
journal, November 2014
- Sultana, Afia; Nakaya, Naoki; Dong, Lijin
- Experimental Neurology, Vol. 261
Elucidation of subfamily segregation and intramolecular coevolution of the olfactomedin-like proteins by comprehensive phylogenetic analysis and gene expression pattern assessment
journal, September 2005
- Zeng, Ling-Chun; Han, Ze-Guang; Ma, Wei-Jun
- FEBS Letters, Vol. 579, Issue 25
Amyloid Fibril Formation by the Glaucoma-Associated Olfactomedin Domain of Myocilin
journal, August 2012
- Orwig, Susan D.; Perry, Christopher W.; Kim, Laura Y.
- Journal of Molecular Biology, Vol. 421, Issue 2-3
The Glaucoma-Associated Olfactomedin Domain of Myocilin Forms Polymorphic Fibrils That Are Constrained by Partial Unfolding and Peptide Sequence
journal, February 2014
- Hill, Shannon E.; Donegan, Rebecca K.; Lieberman, Raquel L.
- Journal of Molecular Biology, Vol. 426, Issue 4
MicroRNAs Shape the Neuronal Landscape
journal, August 2012
- McNeill, Elizabeth; Van Vactor, David
- Neuron, Vol. 75, Issue 3
The many blades of the β-propeller proteins: conserved but versatile
journal, October 2011
- Chen, Cammy K. -M.; Chan, Nei-Li; Wang, Andrew H. -J.
- Trends in Biochemical Sciences, Vol. 36, Issue 10
Novel Sequences Propel Familiar Folds
journal, April 2002
- Jawad, Zahra; Paoli, Massimo
- Structure, Vol. 10, Issue 4
Characterization and differential expression of a human gene family of olfactomedin-related proteins
journal, August 2000
- Kulkarni, Nalini H.; Karavanich, Christa A.; Atchley, William R.
- Genetical Research, Vol. 76, Issue 1
The Stability of Myocilin Olfactomedin Domain Variants Provides New Insight into Glaucoma as a Protein Misfolding Disorder
journal, July 2011
- Burns, J. Nicole; Turnage, Katherine C.; Walker, Chandler A.
- Biochemistry, Vol. 50, Issue 26
Ligands for Glaucoma-Associated Myocilin Discovered by a Generic Binding Assay
journal, November 2013
- Orwig, Susan D.; Chi, Pamela V.; Du, Yuhong
- ACS Chemical Biology, Vol. 9, Issue 2
Rescue of Glaucoma-Causing Mutant Myocilin Thermal Stability by Chemical Chaperones
journal, March 2010
- Burns, J. Nicole; Orwig, Susan D.; Harris, Julia L.
- ACS Chemical Biology, Vol. 5, Issue 5
Docking Server for the Identification of Heparin Binding Sites on Proteins
journal, July 2014
- Mottarella, Scott E.; Beglov, Dmitri; Beglova, Natalia
- Journal of Chemical Information and Modeling, Vol. 54, Issue 7
Noelin-1 is a secreted glycoprotein involved in generation of the neural crest
journal, March 2000
- Barembaum, Meyer; Moreno, Tanya A.; LaBonne, Carole
- Nature Cell Biology, Vol. 2, Issue 4
Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001
- Baker, N. A.; Sept, D.; Joseph, S.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041
The beta bulge: a common small unit of nonrepetitive protein structure.
journal, June 1978
- Richardson, J. S.; Getzoff, E. D.; Richardson, D. C.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 6
Fibronectin Type III-like Domains of Neurofascin-186 Protein Mediate Gliomedin Binding and Its Clustering at the Developing Nodes of Ranvier
journal, October 2011
- Labasque, Marilyne; Devaux, Jérôme J.; Lévêque, Christian
- Journal of Biological Chemistry, Vol. 286, Issue 49
Olfactomedin 1 Interacts with the Nogo A Receptor Complex to Regulate Axon Growth
journal, August 2012
- Nakaya, Naoki; Sultana, Afia; Lee, Hee-Sheung
- Journal of Biological Chemistry, Vol. 287, Issue 44
Cleavage and Oligomerization of Gliomedin, a Transmembrane Collagen Required for Node of Ranvier Formation
journal, February 2007
- Maertens, Barbara; Hopkins, Delana; Franzke, Claus-Werner
- Journal of Biological Chemistry, Vol. 282, Issue 14
A Cellular Assay Distinguishes Normal and Mutant TIGR/Myocilin Protein
journal, November 1999
- Zhou, Z.; Vollrath, D.
- Human Molecular Genetics, Vol. 8, Issue 12
Exploiting the interaction between Grp94 and aggregated myocilin to treat glaucoma
journal, July 2014
- Stothert, Andrew R.; Suntharalingam, Amirthaa; Huard, Dustin J. E.
- Human Molecular Genetics, Vol. 23, Issue 24
Structural basis for misfolding in myocilin-associated glaucoma
journal, December 2014
- Donegan, Rebecca K.; Hill, Shannon E.; Freeman, Dana M.
- Human Molecular Genetics, Vol. 24, Issue 8
ESPript/ENDscript: extracting and rendering sequence and 3D information from atomic structures of proteins
journal, July 2003
- Gouet, P.
- Nucleic Acids Research, Vol. 31, Issue 13
DISPLAR: an accurate method for predicting DNA-binding sites on protein surfaces
journal, February 2007
- Tjong, Harianto; Zhou, Huan-Xiang
- Nucleic Acids Research, Vol. 35, Issue 5
Characterization of extracellular circulating microRNA
journal, May 2011
- Turchinovich, Andrey; Weiz, Ludmila; Langheinz, Anne
- Nucleic Acids Research, Vol. 39, Issue 16
Increased expression of olfactomedin-1 and myocilin in podocytes during puromycin aminonucleoside nephrosis
journal, June 2010
- Bohr, D. C.; Koch, M.; Kritzenberger, M.
- Nephrology Dialysis Transplantation, Vol. 26, Issue 1
Increasing the hydrophobic interaction between terminal W-motifs enhances the stability of Salmonella typhimurium sialidase. A general strategy for the stabilization of β-propeller protein fold
journal, November 2001
- Witarto, Arief Budi; Sode, Koji
- Protein Engineering, Design and Selection, Vol. 14, Issue 11
Targeted Disruption of the Myocilin Gene (Myoc) Suggests that Human Glaucoma-Causing Mutations Are Gain of Function
journal, November 2001
- Kim, B. S.; Savinova, O. V.; Reedy, M. V.
- Molecular and Cellular Biology, Vol. 21, Issue 22
Pancortins interact with amyloid precursor protein and modulate cortical cell migration
journal, September 2012
- Rice, H. C.; Townsend, M.; Bai, J.
- Development, Vol. 139, Issue 21
Olfactomedin-1 activity identifies a cell invasion checkpoint during epithelial-mesenchymal transition in the chick embryonic heart
journal, December 2012
- Lencinas, A.; Chhun, D. C.; Dan, K. P.
- Disease Models & Mechanisms, Vol. 6, Issue 3
A genome-wide association meta-analysis identifies new childhood obesity loci
journal, March 2012
- Bradfield JP, -; Taal HR, -; Timpson NJ, -
- Obesity and metabolism, Vol. 9, Issue 1
Olfactomedin 4 Inhibits Cathepsin C-Mediated Protease Activities, Thereby Modulating Neutrophil Killing of Staphylococcus aureus and Escherichia coli in Mice
journal, July 2012
- Liu, Wenli; Yan, Ming; Liu, Yueqin
- The Journal of Immunology, Vol. 189, Issue 5
Works referencing / citing this record:
Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells
journal, November 2018
- Lynch, Jeffrey M.; Li, Bing; Katoli, Parvaneh
- Journal of Biological Chemistry, Vol. 293, Issue 52
Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between benign and glaucoma-causing mutations
journal, July 2019
- Hill, Shannon E.; Kwon, Michelle S.; Martin, Mackenzie D.
- Journal of Biological Chemistry, Vol. 294, Issue 34
Calcium-ligand variants of the myocilin olfactomedin propeller selected from invertebrate phyla reveal cross-talk with N-terminal blade and surface helices
journal, August 2019
- Hill, Shannon E.; Cho, Hayeon; Raut, Priyam
- Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 9
Design and structural characterisation of olfactomedin-1 variants as tools for functional studies
journal, November 2019
- Pronker, Matti F.; van den Hoek, Hugo; Janssen, Bert J. C.
- BMC Molecular and Cell Biology, Vol. 20, Issue 1
Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability
journal, April 2018
- Goldenzweig, Adi; Goldsmith, Moshe; Hill, Shannon E.
- Molecular Cell, Vol. 70, Issue 2
Design and structural characterisation of olfactomedin-1 variants as tools for functional studies
journal, November 2019
- Pronker, Matti F.; van den Hoek, Hugo; Janssen, Bert J. C.
- BMC Molecular and Cell Biology, Vol. 20, Issue 1