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Title: Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing

Abstract

Peptide loading of major histocompatibility complex class I (MHC-I) molecules is central to antigen presentation, self-tolerance, and CD8+ T-cell activation. TAP binding protein, related (TAPBPR), a widely expressed tapasin homolog, is not part of the classical MHC-I peptide-loading complex (PLC). Using recombinant MHC-I molecules, we show that TAPBPR binds HLA-A*02:01 and several other MHC-I molecules that are either peptide-free or loaded with low-affinity peptides. Fluorescence polarization experiments establish that TAPBPR augments peptide binding by MHC-I. The TAPBPR/MHC-I interaction is reversed by specific peptides, related to their affinity. Mutational and small-angle X-ray scattering (SAXS) studies confirm the structural similarities of TAPBPR with tapasin. These results support a role of TAPBPR in stabilizing peptide-receptive conformation(s) of MHC-I, permitting peptide editing.

Authors:
 [1];  [2];  [1];  [1];  [1];  [3];  [4];  [4];  [5];  [5];  [2];  [1];  [1]
  1. National Inst. of Health (NIH), Bethesda, MD (United States). National Inst. of Allergy and Infectious Diseases. Lab. of Immunology. Molecular Biology Section
  2. National Inst. of Health (NIH), Bethesda, MD (United States). National Inst. of Biomedical Imaging and Bioengineering. Lab. of Cellular Imaging and Macromolecular Biophysics
  3. National Inst. of Health (NIH), Bethesda, MD (United States). National Inst. of Allergy and Infectious Diseases. Office of Cyber Infrastructure and Computational Biology. Bioinformatics and Computational Biosciences Branch
  4. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Drug Evaluation and Research. Lab. of Immunology
  5. Univ. of Oklahoma Health Sciences Center, Oklahoma City, OK (United States). Dept. of Microbiology and Immunology
Publication Date:
Research Org.:
National Institutes of Health (NIH), Bethesda, MD (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Inst. of Health (NIH) (United States)
Contributing Org.:
Food and Drug Administration (FDA), Silver Spring, MD (United States); Univ. of Oklahoma Health Sciences Center, Oklahoma City, OK (United States)
OSTI Identifier:
1242290
Grant/Contract Number:  
AC02-06CH11357; 9 P41 GM103622; AI0000394; EB000008; 1617
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 113; Journal Issue: 8; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
ENGLISH
Subject:
60 APPLIED LIFE SCIENCES; antigen presentation; peptide loading; major histocompatibility complex; protein interactions; SAXS

Citation Formats

Morozov, Giora I., Zhao, Huaying, Mage, Michael G., Boyd, Lisa F., Jiang, Jiansheng, Dolan, Michael A., Venna, Ramesh, Norcross, Michael A., McMurtrey, Curtis P., Hildebrand, William, Schuck, Peter, Natarajan, Kannan, and Margulies, David H. Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing. United States: N. p., 2016. Web. doi:10.1073/pnas.1519894113.
Morozov, Giora I., Zhao, Huaying, Mage, Michael G., Boyd, Lisa F., Jiang, Jiansheng, Dolan, Michael A., Venna, Ramesh, Norcross, Michael A., McMurtrey, Curtis P., Hildebrand, William, Schuck, Peter, Natarajan, Kannan, & Margulies, David H. Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing. United States. https://doi.org/10.1073/pnas.1519894113
Morozov, Giora I., Zhao, Huaying, Mage, Michael G., Boyd, Lisa F., Jiang, Jiansheng, Dolan, Michael A., Venna, Ramesh, Norcross, Michael A., McMurtrey, Curtis P., Hildebrand, William, Schuck, Peter, Natarajan, Kannan, and Margulies, David H. 2016. "Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing". United States. https://doi.org/10.1073/pnas.1519894113. https://www.osti.gov/servlets/purl/1242290.
@article{osti_1242290,
title = {Interaction of TAPBPR, a tapasin homolog, with MHC-I molecules promotes peptide editing},
author = {Morozov, Giora I. and Zhao, Huaying and Mage, Michael G. and Boyd, Lisa F. and Jiang, Jiansheng and Dolan, Michael A. and Venna, Ramesh and Norcross, Michael A. and McMurtrey, Curtis P. and Hildebrand, William and Schuck, Peter and Natarajan, Kannan and Margulies, David H.},
abstractNote = {Peptide loading of major histocompatibility complex class I (MHC-I) molecules is central to antigen presentation, self-tolerance, and CD8+ T-cell activation. TAP binding protein, related (TAPBPR), a widely expressed tapasin homolog, is not part of the classical MHC-I peptide-loading complex (PLC). Using recombinant MHC-I molecules, we show that TAPBPR binds HLA-A*02:01 and several other MHC-I molecules that are either peptide-free or loaded with low-affinity peptides. Fluorescence polarization experiments establish that TAPBPR augments peptide binding by MHC-I. The TAPBPR/MHC-I interaction is reversed by specific peptides, related to their affinity. Mutational and small-angle X-ray scattering (SAXS) studies confirm the structural similarities of TAPBPR with tapasin. These results support a role of TAPBPR in stabilizing peptide-receptive conformation(s) of MHC-I, permitting peptide editing.},
doi = {10.1073/pnas.1519894113},
url = {https://www.osti.gov/biblio/1242290}, journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 8,
volume = 113,
place = {United States},
year = {Thu Feb 11 00:00:00 EST 2016},
month = {Thu Feb 11 00:00:00 EST 2016}
}

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Works referenced in this record:

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Works referencing / citing this record:

TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
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A personal retrospective on the mechanisms of antigen processing
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Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle
journal, July 2018


Crystal structure of a TAPBPR–MHC I complex reveals the mechanism of peptide editing in antigen presentation
journal, October 2017


Structure of the TAPBPR–MHC I complex defines the mechanism of peptide loading and editing
journal, October 2017


The Role of Molecular Flexibility in Antigen Presentation and T Cell Receptor-Mediated Signaling
journal, July 2018


TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
posted_content, November 2018


High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange
posted_content, November 2019


Structural aspects of chaperone-mediated peptide loading in the MHC-I antigen presentation pathway
journal, March 2019


Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection
posted_content, September 2019


Utilizing TAPBPR to promote exogenous peptide loading onto cell surface MHC I molecules
journal, September 2018


TAPBPR mediates peptide dissociation from MHC class I using a leucine lever.
text, January 2018


Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection
journal, December 2019


Utilizing TAPBPR to promote exogenous peptide loading onto cell surface MHC I molecules.
text, January 2018


Peptide exchange on MHC-I by TAPBPR is driven by a negative allostery release cycle
journal, July 2018


Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection
journal, December 2019


TAPBPR mediates peptide dissociation from MHC class I using a leucine lever
posted_content, November 2018


Preferential interaction of MHC class I with TAPBPR in the absence of glycosylation.
journalarticle, January 2019


Proofreading of Peptide—MHC Complexes through Dynamic Multivalent Interactions
journal, February 2017