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Title: Effects of sample treatments on genome recovery via single-cell genomics

It is known that single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we demonstrate that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence in situ hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing.
 [1] ;  [1] ;  [2] ;  [1]
  1. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  2. Univ. of Queensland, Brisbane (Australia)
Publication Date:
OSTI Identifier:
Report Number(s):
Journal ID: ISSN 1751-7362
DOE Contract Number:
AC02-05CH11231; LE120100025; DP120103498
Resource Type:
Journal Article
Resource Relation:
Journal Name: The ISME Journal; Journal Volume: 8; Journal Issue: 12
Nature Publishing Group
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC); Australian Research Council
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES single-cell genomics; sample preservation; FISH