A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis

Larimer, Curtis J.; Winder, Eric M.; Jeters, Robert T.; Prowant, Matthew S.; Nettleship, Ian; Addleman, Raymond S.; Bonheyo, George T.

doi:10.1007/s00216-015-9195-z

Title: A method for rapid quantitative assessment of biofilms with biomolecular staining and image analysis

Journal Article · Mon Dec 07 00:00:00 EST 2015 · Analytical and Bioanalytical Chemistry

DOI:https://doi.org/10.1007/s00216-015-9195-z· OSTI ID:1239474

Larimer, Curtis J. ^[1]; Winder, Eric M. ^[1]; Jeters, Robert T. ^[1]; Prowant, Matthew S. ^[1]; Nettleship, Ian ^[2]; Addleman, Raymond S. ^[1]; Bonheyo, George T. ^[1]

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Univ. of Pittsburgh, Pittsburgh, PA (United States)

Here, the accumulation of bacteria in surface attached biofilms, or biofouling, can be detrimental to human health, dental hygiene, and many industrial processes. A critical need in identifying and preventing the deleterious effects of biofilms is the ability to observe and quantify their development. Analytical methods capable of assessing early stage fouling are cumbersome or lab-confined, subjective, and qualitative. Herein, a novel photographic method is described that uses biomolecular staining and image analysis to enhance contrast of early stage biofouling. A robust algorithm was developed to objectively and quantitatively measure surface accumulation of Pseudomonas putida from photographs and results were compared to independent measurements of cell density. Results from image analysis quantified biofilm growth intensity accurately and with approximately the same precision of the more laborious cell counting method. This simple method for early stage biofilm detection enables quantifiable measurement of surface fouling and is flexible enough to be applied from the laboratory to the field. Broad spectrum staining highlights fouling biomass, photography quickly captures a large area of interest, and image analysis rapidly quantifies fouling in the image.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1239474

Report Number(s):: PNNL-SA-111904; 48240; 48669

Journal Information:: Analytical and Bioanalytical Chemistry, Vol. 408, Issue 3; ISSN 1618-2642

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 13 works

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Recent progress in bio-inspired biofilm-resistant polymeric surfaces Cattò, Cristina; Villa, Federica; Cappitelli, Francesca Critical Reviews in Microbiology, Vol. 44, Issue 5 https://doi.org/10.1080/1040841x.2018.1489369	journal	July 2018
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