A Mo-anode-based in-house source for small-angle X-ray scattering measurements of biological macromolecules

Bruetzel, Linda K.; Fischer, Stefan; Salditt, Annalena; Sedlak, Steffen M.; Nickel, Bert; Lipfert, Jan

doi:10.1063/1.4940936

Title: A Mo-anode-based in-house source for small-angle X-ray scattering measurements of biological macromolecules

Journal Article · Mon Feb 08 00:00:00 EST 2016 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.4940936· OSTI ID:1238284

Bruetzel, Linda K. ^[1]; Fischer, Stefan ^[1]; Salditt, Annalena ^[1]; Sedlak, Steffen M. ^[1]; Nickel, Bert ^[1]; Lipfert, Jan ^[1]

Ludwig Maximilian Univ., Munich (Germany). Dept. of Physics, Nanosystems Initiative Munich, and Center for Nanoscience

We demonstrate the use of a molybdenum-anode-based in-house small-angle X-ray scattering (SAXS) setup to study biological macromolecules in solution. Our system consists of a microfocus X-ray tube delivering a highly collimated flux of 2.5 × 10⁶ photons/s at a beam size of 1.2 × 1.2 mm² at the collimation path exit and a maximum beam divergence of 0.16 mrad. The resulting observable scattering vectors q are in the range of 0.38 Å^–1 down to 0.009 Å^–1 in SAXS configuration and of 0.26 Å^–1 up to 5.7 Å^–1 in wide-angle X-ray scattering (WAXS) mode. To determine the capabilities of the instrument, we collected SAXS data on weakly scattering biological macromolecules including proteins and a nucleic acid sample with molecular weights varying from ~12 to 69 kDa and concentrations of 1.5–24 mg/ml. We report the measured scattering data display a high signal-to-noise ratio up to q-values of ~0.2 Å^–1 allowing for an accurate structural characterization of the samples. Moreover, the in-house source data are of sufficient quality to perform ab initio 3D structure reconstructions that are in excellent agreement with the available crystallographic structures. In addition, measurements for the detergent decyl-maltoside show that the setup can be used to determine the size, shape, and interactions (as characterized by the second virial coefficient) of detergent micelles. This demonstrates that the use of a Mo-anode based in-house source is sufficient to determine basic geometric parameters and 3D shapes of biomolecules and presents a viable alternative to valuable beam time at third generation synchrotron sources.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI ID:: 1238284

Journal Information:: Review of Scientific Instruments, Vol. 87, Issue 2; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 12 works

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