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Title: COPI selectively drives maturation of the early Golgi

Abstract

COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Lastly, our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1236994
Alternate Identifier(s):
OSTI ID: 1236996; OSTI ID: 1258763
Grant/Contract Number:  
AC02-06CH11357; R01 GM104010; T32 GM007183
Resource Type:
Journal Article: Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Name: eLife Journal Volume: 4; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, and Glick, Benjamin S. COPI selectively drives maturation of the early Golgi. United States: N. p., 2015. Web. doi:10.7554/eLife.13232.
Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, & Glick, Benjamin S. COPI selectively drives maturation of the early Golgi. United States. https://doi.org/10.7554/eLife.13232
Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, and Glick, Benjamin S. 2015. "COPI selectively drives maturation of the early Golgi". United States. https://doi.org/10.7554/eLife.13232.
@article{osti_1236994,
title = {COPI selectively drives maturation of the early Golgi},
author = {Papanikou, Effrosyni and Day, Kasey J. and Austin, Jotham and Glick, Benjamin S.},
abstractNote = {COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Lastly, our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.},
doi = {10.7554/eLife.13232},
url = {https://www.osti.gov/biblio/1236994}, journal = {eLife},
issn = {2050-084X},
number = ,
volume = 4,
place = {United States},
year = {Mon Dec 28 00:00:00 EST 2015},
month = {Mon Dec 28 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.7554/eLife.13232

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