COPI selectively drives maturation of the early Golgi
Abstract
COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Lastly, our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Chicago, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1236994
- Alternate Identifier(s):
- OSTI ID: 1236996; OSTI ID: 1258763
- Grant/Contract Number:
- AC02-06CH11357; R01 GM104010; T32 GM007183
- Resource Type:
- Journal Article: Published Article
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Name: eLife Journal Volume: 4; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, and Glick, Benjamin S. COPI selectively drives maturation of the early Golgi. United States: N. p., 2015.
Web. doi:10.7554/eLife.13232.
Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, & Glick, Benjamin S. COPI selectively drives maturation of the early Golgi. United States. https://doi.org/10.7554/eLife.13232
Papanikou, Effrosyni, Day, Kasey J., Austin, Jotham, and Glick, Benjamin S. 2015.
"COPI selectively drives maturation of the early Golgi". United States. https://doi.org/10.7554/eLife.13232.
@article{osti_1236994,
title = {COPI selectively drives maturation of the early Golgi},
author = {Papanikou, Effrosyni and Day, Kasey J. and Austin, Jotham and Glick, Benjamin S.},
abstractNote = {COPI coated vesicles carry material between Golgi compartments, but the role of COPI in the secretory pathway has been ambiguous. Previous studies of thermosensitive yeast COPI mutants yielded the surprising conclusion that COPI was dispensable both for the secretion of certain proteins and for Golgi cisternal maturation. To revisit these issues, we optimized the anchor-away method, which allows peripheral membrane proteins such as COPI to be sequestered rapidly by adding rapamycin. Video fluorescence microscopy revealed that COPI inactivation causes an early Golgi protein to remain in place while late Golgi proteins undergo cycles of arrival and departure. These dynamics generate partially functional hybrid Golgi structures that contain both early and late Golgi proteins, explaining how secretion can persist when COPI has been inactivated. Lastly, our findings suggest that cisternal maturation involves a COPI-dependent pathway that recycles early Golgi proteins, followed by multiple COPI-independent pathways that recycle late Golgi proteins.},
doi = {10.7554/eLife.13232},
url = {https://www.osti.gov/biblio/1236994},
journal = {eLife},
issn = {2050-084X},
number = ,
volume = 4,
place = {United States},
year = {Mon Dec 28 00:00:00 EST 2015},
month = {Mon Dec 28 00:00:00 EST 2015}
}
Web of Science
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