Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite

Vandamme, Dries; Pohl, Philip I.; Beuckels, Annelies; Foubert, Imogen; Brady, Patrick Vane; Muylaert, Koenraad; Hewson, John C.

doi:10.1016/j.biortech.2015.08.042

Title: Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite

Journal Article · Thu Aug 20 00:00:00 EDT 2015 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2015.08.042· OSTI ID:1236222

Vandamme, Dries ^[1]; Pohl, Philip I. ^[2]; Beuckels, Annelies ^[1]; Foubert, Imogen ^[3]; Brady, Patrick Vane ^[2]; Muylaert, Koenraad ^[1]; Hewson, John C. ^[2]

KU Leuven Kulak, Kortrijk (Belgium)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
KU Leuven, Leuven (Belgium)

Alkaline flocculation holds great potential as a low-cost harvesting method for marine microalgae biomass production. Alkaline flocculation is induced by an increase in pH and is related to precipitation of calcium and magnesium salts. In this study, we used the diatom Phaeodactylum tricornutum as model organism to study alkaline flocculation of marine microalgae cultured in seawater medium. Flocculation started when pH was increased to 10 and flocculation efficiency reached 90% when pH was 10.5, which was consistent with precipitation modeling for brucite or Mg(OH)₂. Compared to freshwater species, more magnesium is needed to achieve flocculation (>7.5 mM). Zeta potential measurements suggest that brucite precipitation caused flocculation by charge neutralization. When calcium concentration was 12.5 mM, flocculation was also observed at a pH of 10. Furthermore, zeta potential remained negative up to pH 11.5, suggesting that precipitated calcite caused flocculation by a sweeping coagulation mechanism.

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Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC04-94AL85000; 9.1.1.3_DE-EE0005995

OSTI ID:: 1236222

Alternate ID(s):: OSTI ID: 1250331

Report Number(s):: SAND-2015-6106J; 615250

Journal Information:: Bioresource Technology, Vol. 196, Issue C; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 33 works

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60 APPLIED LIFE SCIENCES
coagulation
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biomass
autoflocculation

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