skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Journal Article · · Nature Methods
DOI:https://doi.org/10.1038/nmeth.3667· OSTI ID:1234715

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-76SF00515
OSTI ID:
1234715
Journal Information:
Nature Methods, Vol. 13; ISSN 1548-7091
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English

References (44)

Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor journal January 2005
RCrane : semi-automated RNA model building journal July 2012
Linking Crystallographic Model and Data Quality journal May 2012
Development of an X-ray pixel detector with multi-port charge-coupled device for X-ray free-electron laser experiments journal March 2014
CrystFEL : a software suite for snapshot serial crystallography journal March 2012
Native-like Photosystem II Superstructure at 2.44 Å Resolution through Detergent Extraction from the Protein Crystal journal November 2014
Single-Molecule Analysis of Translational Dynamics journal July 2012
Grease matrix as a versatile carrier of proteins for serial crystallography journal November 2014
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals journal June 2012
Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography journal February 2014
Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses journal November 2014
Fixed-target protein serial microcrystallography with an x-ray free electron laser journal August 2014
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography journal May 2012
A structural basis for streptomycin-induced misreading of the genetic code journal January 2013
Femtosecond X-ray protein nanocrystallography journal February 2011
Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser journal August 2013
Molecular mechanics of 30S subunit head rotation journal September 2014
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy journal July 2014
XFEL diffraction: developing processing methods to optimize data quality journal January 2015
A Capillary Mixer with Adjustable Reaction Chamber Volume for Millisecond Time-Resolved Studies by Electrospray Mass Spectrometry journal December 2003
The Coherent X-ray Imaging instrument at the Linac Coherent Light Source journal April 2015
Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature journal February 2013
Improvements in serial femtosecond crystallography of photosystem II by optimizing crystal uniformity using microseeding procedures journal July 2015
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein journal December 2014
Potential for biomolecular imaging with femtosecond X-ray pulses journal August 2000
SEM imaging of liquid jets journal June 2009
Structure of the 30S ribosomal subunit journal September 2000
Serial Femtosecond Crystallography of G Protein-Coupled Receptors journal December 2013
Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function journal October 2010
Phaser crystallographic software journal July 2007
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers journal March 2014
Nanoflow electrospinning serial femtosecond crystallography journal October 2012
Physicochemical Properties of Deoxyribonucleic Acid from an Extreme Thermophile journal January 1974
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome journal December 2019
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation journal February 2021
Accurate determination of segmented X-ray detector geometry text January 2015
PHENIX: a comprehensive Python-based system for macromolecular structure solution. text January 2010
Modified Uridines with C5-methylene Substituents at the First Position of the tRNA Anticodon Stabilize U·G Wobble Pairing during Decoding journal May 2008
Features and development of Coot journal March 2010
Crystallographic data processing for free-electron laser sources journal June 2013
Temperature-dependent radiation sensitivity and order of 70S ribosome crystals journal October 2014
Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data journal May 2014
Femtosecond protein nanocrystallography—data analysis methods journal January 2010

Cited By (37)

Viral internal ribosomal entry sites: four classes for one goal: Viral internal ribosomal entry sites journal November 2017
Structure of photosystem II and substrate binding at room temperature journal November 2016
Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin journal April 2017
Graphene-based microfluidics for serial crystallography journal January 2016
Microfluidics: From crystallization to serial time-resolved crystallography journal May 2017
An outlook on using serial femtosecond crystallography in drug discovery journal May 2019
Watching proteins function with time-resolved x-ray crystallography journal August 2017
Aminoglycoside ribosome interactions reveal novel conformational states at ambient temperature journal August 2018
Protein crystals IR laser ablated from aqueous solution at high speed retain their diffractive properties: applications in high-speed serial crystallography journal November 2017
3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating journal August 2019
Successful sample preparation for serial crystallography experiments journal November 2019
A convolutional neural network-based screening tool for X-ray serial crystallography journal April 2018
The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source journal February 2019
XANES and EXAFS of dilute solutions of transition metals at XFELs journal August 2019
Crystallography on a chip – without the chip: sheet-on-sheet sandwich journal October 2018
Sample delivery for serial crystallography at free-electron lasers and synchrotrons journal January 2019
Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography journal January 2019
Strategies for sample delivery for femtosecond crystallography journal February 2019
A guide to sample delivery systems for serial crystallography journal August 2019
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography text January 2016
Aerodynamically stabilized Taylor cone jets text January 2019
Recent Advances in Ultrafast Structural Techniques journal April 2019
Polysaccharide-Based Injection Matrix for Serial Crystallography journal May 2020
Double-flow focused liquid injector for efficient serial femtosecond crystallography journal March 2017
Mix-and-inject XFEL crystallography reveals gated conformational dynamics during enzyme catalysis journal December 2019
Aerodynamically stabilized Taylor cone jets journal September 2019
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography text January 2016
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals journal February 2020
Strategies for sample delivery for femtosecond crystallography text January 2019
Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature journal April 2019
Structure of the 30S ribosomal decoding complex at ambient temperature journal August 2018
Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser journal November 2016
Conformational sampling and kinetics changes across a non-Arrhenius break point in the enzyme thermolysin journal January 2020
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography journal August 2016
Emerging Roles of Mitochondrial Ribosomal Proteins in Plant Development journal December 2017
Role of Computational Methods in Going beyond X-ray Crystallography to Explore Protein Structure and Dynamics journal October 2018
Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources journal March 2019

Related Subjects