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Title: Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

Abstract

Methane is a feedstock of interest for the future, both from natural gas and from renewable biogas sources. Methanotrophic bacteria have the potential to enable commercial methane bioconversion to value-added products such as fuels and chemicals. A strain of interest for such applications is Methylomicrobium buryatense 5GB1, due to its robust growth characteristics. But, to take advantage of the potential of this methanotroph, it is important to generate comprehensive bioreactor-based datasets for different growth conditions to compare bioprocess parameters. The datasets of growth parameters, gas utilization rates, and products (total biomass, extracted fatty acids, glycogen, excreted acids) were obtained for cultures of M. buryatense 5GB1 grown in continuous culture under methane limitation and O2 limitation conditions. Additionally, experiments were performed involving unrestricted batch growth conditions with both methane and methanol as substrate. All four growth conditions show significant differences. The most notable changes are the high glycogen content and high formate excretion for cells grown on methanol (batch), and high O2:CH4 utilization ratio for cells grown under methane limitation. The results presented here represent the most comprehensive published bioreactor datasets for a gamma-proteobacterial methanotroph. This information shows that metabolism by M. buryatense 5GB1 differs significantly for each of the fourmore » conditions tested. O2 limitation resulted in the lowest relative O2 demand and fed-batch growth on methane the highest. Future studies are needed to understand the metabolic basis of these differences. However, these results suggest that both batch and continuous culture conditions have specific advantages, depending on the product of interest.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1618796
Alternate Identifier(s):
OSTI ID: 1233686
Report Number(s):
NREL/JA-5100-65607
Journal ID: ISSN 1475-2859; 182; PII: 372
Grant/Contract Number:  
AR0000350; AC36-08GO28308
Resource Type:
Journal Article: Published Article
Journal Name:
Microbial Cell Factories
Additional Journal Information:
Journal Name: Microbial Cell Factories Journal Volume: 14 Journal Issue: 1; Journal ID: ISSN 1475-2859
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; methanotroph; bioreactors; continuous culture; methane; gas-to-liquid

Citation Formats

Gilman, Alexey, Laurens, Lieve M., Puri, Aaron W., Chu, Frances, Pienkos, Philip T., and Lidstrom, Mary E. Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1. United Kingdom: N. p., 2015. Web. doi:10.1186/s12934-015-0372-8.
Gilman, Alexey, Laurens, Lieve M., Puri, Aaron W., Chu, Frances, Pienkos, Philip T., & Lidstrom, Mary E. Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1. United Kingdom. https://doi.org/10.1186/s12934-015-0372-8
Gilman, Alexey, Laurens, Lieve M., Puri, Aaron W., Chu, Frances, Pienkos, Philip T., and Lidstrom, Mary E. 2015. "Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1". United Kingdom. https://doi.org/10.1186/s12934-015-0372-8.
@article{osti_1618796,
title = {Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1},
author = {Gilman, Alexey and Laurens, Lieve M. and Puri, Aaron W. and Chu, Frances and Pienkos, Philip T. and Lidstrom, Mary E.},
abstractNote = {Methane is a feedstock of interest for the future, both from natural gas and from renewable biogas sources. Methanotrophic bacteria have the potential to enable commercial methane bioconversion to value-added products such as fuels and chemicals. A strain of interest for such applications is Methylomicrobium buryatense 5GB1, due to its robust growth characteristics. But, to take advantage of the potential of this methanotroph, it is important to generate comprehensive bioreactor-based datasets for different growth conditions to compare bioprocess parameters. The datasets of growth parameters, gas utilization rates, and products (total biomass, extracted fatty acids, glycogen, excreted acids) were obtained for cultures of M. buryatense 5GB1 grown in continuous culture under methane limitation and O2 limitation conditions. Additionally, experiments were performed involving unrestricted batch growth conditions with both methane and methanol as substrate. All four growth conditions show significant differences. The most notable changes are the high glycogen content and high formate excretion for cells grown on methanol (batch), and high O2:CH4 utilization ratio for cells grown under methane limitation. The results presented here represent the most comprehensive published bioreactor datasets for a gamma-proteobacterial methanotroph. This information shows that metabolism by M. buryatense 5GB1 differs significantly for each of the four conditions tested. O2 limitation resulted in the lowest relative O2 demand and fed-batch growth on methane the highest. Future studies are needed to understand the metabolic basis of these differences. However, these results suggest that both batch and continuous culture conditions have specific advantages, depending on the product of interest.},
doi = {10.1186/s12934-015-0372-8},
url = {https://www.osti.gov/biblio/1618796}, journal = {Microbial Cell Factories},
issn = {1475-2859},
number = 1,
volume = 14,
place = {United Kingdom},
year = {Mon Nov 16 00:00:00 EST 2015},
month = {Mon Nov 16 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at https://doi.org/10.1186/s12934-015-0372-8

Citation Metrics:
Cited by: 70 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1 : Core metabolism of methane in Group I methanotrophs. pMMO particulate methane monooxygenase, MeDH methanol dehydrogenase, RuMP cycle Ribulose monophosphate cycle

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Works referenced in this record:

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Works referencing / citing this record:

Hydrogen Oxidation Influences Glycogen Accumulation in a Verrucomicrobial Methanotroph
journal, August 2019


A micro-jet array for economic intensification of gas transfer in bioreactors
journal, October 2018


A modular approach for high-flux lactic acid production from methane in an industrial medium using engineered Methylomicrobium buryatense 5GB1
journal, April 2018


Biological conversion of methane to methanol through genetic reassembly of native catalytic domains
journal, April 2019


Industrial biomanufacturing: The future of chemical production
journal, January 2017


Future Applications of Biotechnology to the Energy Industry
journal, February 2016


Fatty Acid Biosynthesis Pathways in Methylomicrobium buryatense 5G(B1)
journal, January 2017


Oxygen-limited metabolism in the methanotroph Methylomicrobium buryatense 5GB1C
journal, January 2017


Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.