Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module

Guan, Xiaoyang; Chaffey, Patrick K.; Zeng, Chen; Greene, Eric R.; Chen, Liqun; Drake, Matthew R.; Chen, Claire; Groobman, Ari; Resch, Michael G.; Himmel, Michael E.; Beckham, Gregg T.; Tan, Zhongping

doi:10.1039/C5SC02636A

Title: Molecular-Scale Features that Govern the Effects of O-Glycosylation on a Carbohydrate-Binding Module

Journal Article · Mon Sep 21 00:00:00 EDT 2015 · Chemical Science

DOI:https://doi.org/10.1039/C5SC02636A· OSTI ID:1233680

Guan, Xiaoyang ^[1]; Chaffey, Patrick K. ^[1]; Zeng, Chen ^[1]; Greene, Eric R. ^[1]; Chen, Liqun ^[1]; Drake, Matthew R. ^[1]; Chen, Claire ^[1]; Groobman, Ari ^[1]; Resch, Michael G. ^[2]; Himmel, Michael E. ^[2]; Beckham, Gregg T. ^[2]; Tan, Zhongping ^[1]

Univ. of Colorado, Boulder, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

The protein glycosylation is a ubiquitous post-translational modification in all kingdoms of life. Despite its importance in molecular and cellular biology, the molecular-level ramifications of O-glycosylation on biomolecular structure and function remain elusive. Here, we took a small model glycoprotein and changed the glycan structure and size, amino acid residues near the glycosylation site, and glycosidic linkage while monitoring any corresponding changes to physical stability and cellulose binding affinity. The results of this study reveal the collective importance of all the studied features in controlling the most pronounced effects of O-glycosylation in this system. This study suggests the possibility of designing proteins with multiple improved properties by simultaneously varying the structures of O-glycans and amino acids local to the glycosylation site.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1233680

Report Number(s):: NREL/JA-5100-65609

Journal Information:: Chemical Science, Vol. 6, Issue 12; Related Information: Chemical Science; ISSN 2041-6520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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