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Title: The role of carbonic anhydrase in C 4 photosynthesis

Current pressures on the global food supply have accelerated the urgency for a second green revolution using novel and sustainable approaches to increase crop yield and efficiency. This proposal outlines experiments to address fundamental questions regarding the biology of C 4 photosynthesis, the method of carbon fixation utilized by the most productive food, feed and bioenergy crops. Carbonic anhydrase (CA) has been implicated in multiple cellular functions including nitrogen metabolism, water use efficiency, and photosynthesis. CA catalyzes the first dedicated step in C 4 photosynthesis, the hydration of CO 2 into bicarbonate, and is potentially rate limiting in C 4 grasses. Using insertional mutagenesis, we have generated CA mutants in maize, and propose the characterization of these mutants using phenotypic, physiological, and transcriptomic profiling to assay the plant’s response to altered CA activity. In addition, florescent protein tagging experiments will be employed to study the subcellular localization of CA paralogs, providing critical data for modeling carbon fixation in C 4 plants. Finally, I propose parallel experiments in Setaria viridis to explore its relevance as model C 4 grass. Using a multifaceted approach, this proposal addresses important questions in basic biology, as well as the need for translation research in responsemore » to looming global food challenges.« less
Authors:
 [1]
  1. Life Sciences Research Foundation, Baltimore, MD (United States)
Publication Date:
OSTI Identifier:
1233447
Report Number(s):
SC0008510FinalReport
DOE Contract Number:
SC0008510
Resource Type:
Technical Report
Research Org:
Life Sciences Research Foundation, Baltimore, MD (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES